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ASCP MLT Exam: Practice Questions

Number of questions: 500

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About the ASCP MLT exam

The ASCP Medical Laboratory Technician (MLT) exam assesses the knowledge required for intermediate-level medical laboratory roles. The American Society for Clinical Pathology (ASCP) administers the exam.

The exam consists of 100 multiple-choice questions and has a 2.5-hour time limit. Exam topics include blood banking, urinalysis, chemistry, hematology, immunology, microbiology, and laboratory procedures.

The exam fee is $225.

About these practice questions

These practice questions will help prepare you for the ASCP MLT exam.

This page contains 500 practice questions divided into the seven sections of the exam: 1. Blood banking, 2. Urinalysis and other body fluids, 3. Chemistry, 4. Hematology, 5. Immunology, 6. Microbiology, and 7. Laboratory operations.

All questions have been carefully designed to mimic the questions on the real exam, to help you prepare and get a passing grade.

Sections

  1. Blood banking
  2. Urinalysis and other body fluids
  3. Chemistry
  4. Hematology
  5. Immunology
  6. Microbiology
  7. Laboratory operations

Section 1: Blood banking

1.1) Which effect refers to the phenomenon where antigen expression is stronger in homozygous genotypes than in heterozygous genotypes?
  1. dosage
  2. homozygous
  3. position
  4. prozone
Answers are locked. Please click here to buy them. 1.2) At which temperature do warm antibodies react with red blood cells?
  1. 15°C
  2. 21°C
  3. 37°C
  4. 55°C
Answers are locked. Please click here to buy them. 1.3) Which IgG subclasses are most efficient at activating complement?
  1. IgG1 and IgG3
  2. IgG1 and IgG4
  3. IgG2 and IgG3
  4. IgG2 and IgG4
Answers are locked. Please click here to buy them. 1.4) Which of these antibodies is clinically insignificant?
  1. Anti-Fya
  2. Anti-Jka
  3. Anti-P1
  4. Anti-s
Answers are locked. Please click here to buy them. 1.5) Which class are most Lewis antibodies?
  1. IgA
  2. IgD
  3. IgG
  4. IgM
Answers are locked. Please click here to buy them. 1.6) Which IgG subclass is most abundant?
  1. IgG1
  2. IgG2
  3. IgG3
  4. IgG4
Answers are locked. Please click here to buy them. 1.7) Which antibody is only found in people with the Bombay blood group?
  1. Anti-D
  2. Anti-F
  3. Anti-H
  4. Anti-I
Answers are locked. Please click here to buy them. 1.8) Which maternal antibody is most commonly responsible for causing fetal neonatal alloimmune thrombocytopenia (FNAIT)?
  1. Anti-A₁
  2. Anti-Fy(a)
  3. Anti-HPA-1a
  4. Anti-Rh(D)
Answers are locked. Please click here to buy them. 1.9) Which solution is used to prepare washed red blood cells?
  1. Acetic acid
  2. Ethanol
  3. Normal saline
  4. Sodium hydroxide
Answers are locked. Please click here to buy them. 1.10) Which of these is a plasma derivative?
  1. Albumin
  2. Platelets
  3. Red blood cells
  4. White blood cells
Answers are locked. Please click here to buy them. 1.11) What is the purpose of the rosette test?
  1. To detect fetomaternal hemorrhage in postpartum women
  2. To screen for red blood cell antibodies in donated blood
  3. To screen for the West Nile virus in donated blood
  4. To test for blood group incompatibility before hematopoietic stem cell transplantation
Answers are locked. Please click here to buy them. 1.12) Which of these situations could result in hemolytic disease of the newborn?
  1. Rh+ mother, Rh+ fetus
  2. Rh+ mother, Rh− fetus
  3. Rh− mother, Rh+ fetus
  4. Rh− mother, Rh− fetus
Answers are locked. Please click here to buy them. 1.13) Which Vacutainer tube is preferred for blood bank tests?
  1. Black
  2. Gray
  3. Green
  4. Pink
Answers are locked. Please click here to buy them. 1.14) Refer to the pedigree chart below. How many of the four children would be expected to have type AB blood?
  1. 1
  2. 2
  3. 3
  4. 4
Answers are locked. Please click here to buy them. 1.15) A patient has hemophilia A and needs injections of factor VIII. However, the patient's body has developed inhibitors to human factor VIII. Which form of factor VIII could be given to this patient instead?
  1. Activated
  2. Canine
  3. Porcine
  4. Recombinant
Answers are locked. Please click here to buy them. 1.16) How does anti-D immunoglobulin prevent Rhesus disease?
  1. By binding and neutralizing anti-D
  2. By blocking H1 receptors, thereby stopping anti-D from reaching its target
  3. By blocking the IgD antigen-binding site
  4. By destroying fetal red blood cells in the mother's bloodstream
Answers are locked. Please click here to buy them. 1.17) How much does one unit of whole blood increase the hematocrit of an average adult?
  1. 3%
  2. 6%
  3. 9%
  4. 12%
Answers are locked. Please click here to buy them. 1.18) In blood transfusions, what kind of red cells are given to patients who have a severe allergy to standard red cells?
  1. Frozen
  2. Irradiated
  3. Synthetic
  4. Washed
Answers are locked. Please click here to buy them. 1.19) Which of the following parasites causes transfusion-associated infections?
  1. Ancylostoma duodenale
  2. Giardia lamblia
  3. Plasmodium malariae
  4. Taenia saginata
Answers are locked. Please click here to buy them.

Section 2: Urinalysis and other body fluids

Section 3: Chemistry

Section 4: Hematology

Section 5: Immunology

Section 6: Microbiology

Section 7: Laboratory operations

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