1) Which type of anemia is associated with an increased reticulocyte count?
Aplastic
Hemolytic
Iron-deficiency
Pernicious
2) A patient asks a phlebotomist if they are allowed to take medication before a fasting blood test. What is the best response?
"It is best to skip today’s dose."
"No, you cannot take medication before this test."
"Yes, unless your doctor told you not to take it."
"Yes, you can take your medication."
3) For which Vacutainer tube is the blood-to-additive ratio most critical?
Green
Lavender
Light blue
Red
4) A lab technician has 1 litre of 85% alcohol. How much water must the technician add to the alcohol to dilute it to 70%?
214 mL
528 mL
1,214 mL
1,528 mL
5) What is the formula for calculating a percent w/w solution?
(solute mass / solution mass ) × 100
(solute mass / solution volume) × 100
(solution mass / solute mass) × 100
(solution mass / solute volume) × 100
6) Calculate the %m/m of a solution that has 30 grams of solute and 90 grams of solvent.
8.3%
9.1%
11%
25%
7) What is the molarity of a 3 L solution that contains 1.2 moles?
0.4
1.8
2.5
3.6
8) You dilute a sample four times using a dilution factor of 20 each time. What is the final dilution?
1/20
1/80
1/8,000
1/160,000
9) A lab assistant makes a 1/6 dilution of serum in water. The assistant then performs a glucose test on the diluted serum. The result is 4.0 mmol/L. What result should the lab assistant report to the doctor?
0.66 mmol/L
4.0 mmol/L
24.0 mmol/L
40.0 mmol/L
10) Attending workshops and regularly updating your knowledge is an example of:
continuing professional development
knowledge and competency mapping
performance appraisal
strategic succession planning
11) What is the term for the obligation to keep health information private?
Accountability
Confidentiality
Privacy
Security
12) What is the term for an individual's agreement to the processing of their personal data?
Accountability
Consent
Openness
Safeguarding
13) A nurse overhears two clinicians talking about a patient. What kind of risk is this?
Accountability
Infection
Privacy
Security
14) A healthcare worker witnesses a manager intentionally breaching a patient's confidentiality. What should the healthcare worker do?
Ignore the breach
Notify the local news station
Notify the patient about the breach
Report it to a senior manager
15) Which of these is a barrier to effective written communication?
Clear subject headings
Concise sentences
Professional tone
Technical jargon
16) Which of these is an example of passive-aggressive behaviour?
Changing the subject when uncomfortable topics arise
Complaining about someone behind their back
Sighing and tutting instead of saying what's wrong
Using veiled threats to control a person's behaviour
17) Leaning forward demonstrates:
confusion
honesty
impatience
interest
18) When communicating with someone from a different culture, it is important to:
make assumptions about their beliefs and values
speak louder and slower to ensure understanding
be respectful of their cultural differences
avoid using humour, as it may be misinterpreted
19) When unsure of a patient’s preferred pronouns, what is the most appropriate action?
Ask a family member of the person
Ask the person what pronouns they prefer
Decide based on a person’s appearance
Use the pronoun of the person’s appearance
20) A patient gets angry at you and raises their voice. What is the best response?
Calmly acknowledge their concern
File a complaint with HR
Raise your voice back
Walk away without saying anything
21) Sitting with your arms crossed conveys:
concern
interest
resistance
sincerity
22) Which of these is an example of non-verbal communication?
Asking a patient to confirm her name
Nodding to show you agree with someone
Saying "No" to a patient
Writing a note to your manager
23) How can you show empathy to a nervous patient?
Distract the patient by talking about your life
Explain the procedure in simple terms
Listen to their concerns
Tell the patient not to worry
24) A colleague is distraught because her cat has died. Which of the following actions would be appropriate?
Ask her to leave the workplace
Offer to share some of her workload
Report the incident to the regulatory body
Tell her to act more professionally
25) A mother arrives at the clinic with her 8-year-old child. The mother does not understand English well. What is the most appropriate action?
Ask a family member or friend to interpret
Ask a trained medical interpreter to help
Use a bilingual staff member
Use simple words and avoid giving detailed explanations
26) Which of these is a barrier to effective communication?
A clear message
Background noise
Emotional intelligence
Eye contact
27) When you are actively listening, where should you look?
At the clock
At the person speaking
At the work in front of you
At your notes
28) A dying patient says, "I’m scared." The most appropriate response is:
“It's normal, try not to think about it.”
“Let’s focus on something positive.”
“Would you like to talk about it?”
“You don’t need to be scared.”
29) A patient becomes increasingly angry and shouts, "I've been waiting here for 6 hours and the doctor still hasn't seen me!" The nurse replies, “I see why that's frustrating. Let me see what I can do.” Which de-escalation technique has the nurse used?
Body language
Offering a choice
Setting boundaries
Validation
30) What should you do if a patient asks why a test has been ordered?
Give a simple explanation
Guess based on the most likely diagnosis
Refer them to their doctor
Tell the patient you are not allowed to answer
31) For which type of patient would an interpreter be useful?
Blind
Intellectually disabled
Non-English speaking
Pediatric
32) What is the best way to confirm a patient has understood your instructions?
Ask the patient to repeat the instructions back to you
Ask, "Did you understand the instructions?"
Ask, "Do you have any questions?"
Have the patient sign a form stating they understood the instructions
33) Which of these is an example of constructive feedback?
"I really enjoy working with you."
"Please remember to mix the tubes after collection."
"Thanks for all your help."
"You have no idea what you're doing. Why can't you do your job properly?"
34) What is the justice pillar of medical ethics?
The duty to do good
The duty to not harm the patient
The respect for the patient’s right to self-determination
The treatment of all people equally and equitably
35) Two patients come to the emergency room and are treated in order of priority based on the severity of their injuries. With which ethical principle is the treatment order consistent?
Beneficence
Justice
Preservation of life
Right to care
36) In the context of patient rights, the principle of autonomy is best described as:
self-awareness
self-governance
self-promotion
selfishness
37) The statement "All tests performed should serve the patient's interests" exemplifies which ethical principle?
Autonomy
Beneficence
Justice
Respect for persons
38) You see a doctor acting unethically. Your first course of action should be to tell:
the hospital director
the medical board
the nursing board
your supervisor
39) What are the four pillars of medical ethics?
Beneficence, non-maleficence, justice, and autonomy
Dignity, privacy, compassion, and accountability
Professionalism, consequentialism, trust, and responsibility
Professionalism, sensitivity, integrity, and confidentiality
40) The attributes of good judgment, respecting patients' rights, and not harming anyone intentionally are all examples of:
competency statements
ethical standards
morals
rules
41) Which pillar of medical ethics is the patient’s right to make decisions about their healthcare?
Autonomy
Beneficence
Justice
Nonmaleficence
42) The duty to "do no harm" is also referred to as:
autonomy
beneficence
justice
non-maleficence
43) On which ethical principle is informed consent based?
Autonomy
Beneficence
Fidelity
Veracity
44) A patient learns best by watching videos that explain their condition. This suggests they are a(n) __________ learner.
auditory
kinesthetic
reading/writing
visual
45) What learning process involves thinking about experiences and learning from them?
Classical conditioning
Concept learning
Implicit learning
Reflective practice
46) Which of these is a key function of a laboratory information system (LIS)?
Monitoring laboratory temperatures
Performing diagnostic tests
Tracking specimens and test results
Training laboratory staff
47) When answering the phone, you should state your name and:
"How are you today?"
the name of your facility
your facility's opening hours
your phone number
48) What is a panic value?
A result that is much higher than normal
A result that is much lower than normal
A wrong result, meaning the test must be repeated
An extremely abnormal result that indicates a life-threatening condition
49) Which coagulation pathway is also known as the contact activation pathway?
Common pathway
Extrinsic pathway
Intrinsic pathway
Tissue factor pathway
50) Insulin stimulates the conversion of glucose into:
galactose
glucagon
glycogen
sucrose
51) Which autoantibody is against the Fc portion of IgG?
Anti-La
Anti-Ro
Anti-nuclear antibody
Rheumatoid factor
52) What is the end product of purine metabolism?
Allantoin
Urea
Uric acid
Xanthine
53) What does angiotensin-converting enzyme convert angiotensin I to?
Aldosterone
Angiotensin II
Angiotensin III
Renin
54) A patient with a severe respiratory infection develops respiratory acidosis. How do the kidney tubules help restore normal blood pH?
Excrete a larger volume of urine
Increase the glomerular filtration rate
Secrete more hydrogen ions and reabsorb more bicarbonate ions
Secrete more sodium ions and reabsorb more potassium ions
55) Which component forms the recognition unit of the classical complement pathway?
C1q
C1r
C1s
C1t
56) The three types of ketone bodies are acetone, acetoacetic acid, and __________ acid.
3-hydroxybutyric
acetic
lactic
oxaloacetic
57) A boy has large bruises without any apparent injury. His platelet count is low but his other CBC parameters are normal. He has no family history of hemorrhagic abnormalities or thrombocytopenia. What is the most likely diagnosis?
Hemolytic uremic syndrome
Immune thrombocytopenic purpura
May-Hegglin anomaly
Von Willebrand disease
58) What is the major health risk for patients who have atrial fibrillation?
Bleeding problems
Bundle branch block
Hypertension
Stroke
59) Which type of jaundice is caused by a blockage in the bile duct?
Hepatocellular
Neonatal
Post-hepatic
Pre-hepatic
60) Which X-linked recessive disease results in the absence of mature B cells?
Chronic granulomatous disease
Wiskott–Aldrich syndrome
X-linked agammaglobulinemia
X-linked lymphoproliferative disease
61) What is ischemia?
A restriction in blood supply to tissues
Persistent elevation of blood pressure in the arteries
Tissue death due to inadequate blood supply to the affected area
When the heart is unable to pump sufficient blood to meet the body's needs
62) Which disease is characterised by hyperthyroidism, an enlarged thyroid, and exophthalmos?
Graves' disease
Hashimoto's disease
Myasthenia gravis
Myxedema
63) What are the main laboratory findings in nephrotic syndrome?
Haemoglobinuria, hypernatremia, and thrombocytopenia
Ketonuria, hyperkalemia, and hypoglycemia
Proteinuria, hyperlipidemia, and hypoalbuminemia
Pyuria, hyperglycemia, and leukopenia
64) Grade 1 tumours grow ________ and have cells that look _________.
quickly, abnormal
quickly, normal
slowly, abnormal
slowly, normal
65) DNA polymerase can synthesize:
DNA in the 3' to 5' direction
DNA in the 5' to 3' direction
RNA in the 3' to 5' direction
RNA in the 5' to 3' direction
66) What is the purpose of the polymerase chain reaction (PCR)?
To create copies of a DNA sequence
To separate DNA fragments by size
To translate a DNA sequence into protein
To translate an RNA sequence into DNA
67) When evaluating a blood smear under a microscope, a lab technician sees a small white blood cell with no cytoplasmic granules. It has a large, round nucleus that takes up most of the cell. Which cell is it most likely to be?
Eosinophil
Lymphocyte
Monocyte
Neutrophil
68) Plasma is obtained by centrifuging:
a red top tube
a tube containing an anticoagulant
an SST
clotted blood
69) Why are bacterial smears heat-fixed before staining?
To ensure the smears can be cut into thin slices
To make the cell walls permeable so the stain can penetrate
To prevent cells from being washed off during staining
To provide a warm temperature for the bacteria to grow
70) Generally, at what pH are fixatives most effective?
3
5
7
9
71) What is the correct order of steps to process tissue containing calcium?
Intracellular clefts and vacuoles in skeletal muscle
Lines across the tissue parallel to the knife edge
73) What is the counterstain in Gram's technique?
Acetone-alcohol
Crystal violet
Iodine
Safranin
74) Which stain is commonly used as an initial step in bacterial identification?
Gram stain
Hematoxylin and eosin stain
Papanicolaou stain
Wright's stain
75) Which stain is most commonly used for peripheral blood smears?
Gram stain
Hematoxylin and eosin
Papanicolaou stain
Wright-Giemsa stain
76) After Wright’s staining, red blood cells look too red and nuclei are faint. What could be the cause?
Inadequate washing
Prolonged staining
The pH of the buffer was too high
The stain/buffer mixture was too acidic
77) Which of these quality control results is acceptable for the triple sugar iron test?
Escherichia coli: yellow slant, yellow butt, no H₂S produced
Pseudomonas aeruginosa: yellow slant, yellow butt, H₂S produced
Salmonella enterica: red slant, red butt, no H₂S produced
Shigella sonnei: red slant, red butt, H₂S produced
78) A minimum inhibitory concentration (MIC) assay is performed on an organism. Given the results below, what is the minimum inhibitory concentration of the organism?
Antibiotic concentration (μg/mL)
64
32
16
8
4
2
0
Result
Clear
Clear
Clear
Turbid
Turbid
Turbid
Turbid
2 μg/mL
4 μg/mL
8 μg/mL
16 μg/mL
79) A bacterium can use peptones both aerobically and anaerobically, but cannot ferment glucose, lactose, or sucrose. What result would you expect on a triple sugar iron (TSI) test?
A/A
K/A
K/K
K/NC
80) Which of these organisms can be used as a positive control for the spot indole test?
Enterobacter cloacae
Escherichia coli
Salmonella enterica
Shigella sonnei
81) Which incubation condition is used to grow Streptococcus pneumoniae?
At 35–37°C on MacConkey agar with 20% H₂
At 35–37°C on blood agar with 5−10% CO₂
At 42°C on Sabouraud agar under anaerobic conditions
At 42°C on chocolate agar under microaerophilic conditions
82) A Neisseria species is isolated from a patient sample. A carbohydrate utilisation test is performed. The results are as follows.
Test
Result
Glucose
+
Maltose
−
Sucrose
−
Lactose
−
Based on these results, which Neisseria species is in the sample?
N. gonorrhoeae
N. lactamica
N. meningitidis
N. sicca
83) Which bacteria is consistent with the results below?
Agar
Result
Blood agar
Beta-hemolytic colonies
MacConkey agar
Colourless colonies
Cetrimide agar
Green colonies
Escherichia coli
Pseudomonas aeruginosa
Staphylococcus aureus
Streptococcus pyogenes
84) A man cuts his hand while doing yard work. The wound becomes infected. A Gram stain reveals the wound contains gram-positive cocci and gram-negative bacilli. Which media can be used to selectively isolate each organism?
Columbia-CNA and chocolate
KV-laked agar and Thayer-Martin
PEA and MacConkey
Sheep blood and chocolate
85) Which bacterium is consistent with the results below?
Agar
Result
Blood agar
Non-hemolytic colonies
MacConkey agar
Pink colonies
Eosin methylene blue agar
Metallic green colonies
Escherichia coli
Salmonella enterica
Staphylococcus aureus
Streptococcus pneumoniae
86) A stool specimen is inoculated onto xylose lysine deoxycholate (XLD) agar. Red colonies form, some of which have black centres. Which bacterium is the most likely?
Escherichia coli
Salmonella
Shigella
Yersinia
87) Which bacterium is consistent with the results below?
Agar
Result
Blood agar
Beta-hemolytic colonies
MacConkey agar
No growth
Mannitol salt agar
Yellow colonies
Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
Streptococcus pyogenes
88) Which of these bacteria is a gram-negative, aerobic, oxidase-positive, motile rod?
Escherichia coli
Pseudomonas aeruginosa
Salmonella enterica
Staphylococcus aureus
89) A 50-year-old inpatient develops a catheter-associated urinary tract infection while in hospital. The urine culture grows >100,000 oxidase-positive gram-negative bacilli. What is the most likely organism?
Enterococcus faecalis
Escherichia coli
Klebsiella aerogenes
Pseudomonas aeruginosa
90) The doctor performs two blood cultures, a sputum culture, a urine culture, and a chest x-ray. All tests are negative except for one blood culture, which grows coagulase-negative staphylococci. These organisms are:
an indication that the patient has meningitis
an indication that the patient has sepsis
most likely a contaminant
most likely a hospital-acquired infection
91) Which bacteria does Löwenstein-Jensen medium isolate?
Escherichia
Hemophilus
Listeria
Mycobacterium
92) Which culture medium is most appropriate for growing gram-negative enteric bacteria?
Blood agar
Chocolate agar
MacConkey agar
Sabouraud agar
93) Which agar isolates and differentiates species of Salmonella and Shigella?
Hektoen enteric
Chocolate
CLED
Thayer Martin
94) A representative sample of uninoculated culture media is incubated for 2–5 days at 35–37°C as part of sterility testing. What is the expected result?
A change in the media's colour
Growth across the entire agar plate
Individual colonies
No microbial growth
95) What is the Gram stain appearance of Escherichia coli?
Gram-negative coccus
Gram-negative rod
Gram-positive coccus
Gram-positive rod
96) A lab technician performs Gram staining of a urine specimen. Under a microscope, the lab technician sees gram-negative cocci in chains. What mistake did the lab technician make?
Forgot to bring the urine specimen to room temperature
Performed the safranin counterstain too quickly
Skipped the iodine step
Used too much crystal violet
97) In Gram staining, which of these situations can cause gram-negative cells to appear colourless?
Omission of the counterstain step
Omission of the iodine treatment step
Over-decolourising
Under-decolourising
98) Which of these is a step in Gram staining?
Chill the specimen on a refrigerated plate
Cover the specimen with iodine for 1 minute
Infiltrate the specimen with paraffin wax
Soak the specimen in an organic solvent such as xylene
99) Which structure in bacterial cells determines whether the bacteria appear gram-positive or gram-negative?
Capsule
Cell wall
Flagella
Ribosome
100) A lab technician is performing a Gram stain. After flooding the slide with crystal violet and letting the slide stand for approximately one minute, the technician should rinse the slide with:
acetone
ethanol
iodine
water
101) In a nitrate reduction test, what colour appears when reagents A and B are added to a control tube with no bacteria?
Blue
Red
Yellow
The tube remains colourless
102) In PCR testing for infectious agents, what do low Ct values indicate?
High viral load
Late infection stage
Low concentration of the target nucleic acid
Low infectivity
103) What does colourimetric analysis measure?
The colour change of a solution during heating
The concentration of a substance in a solution
The light emitted by a solution without external light
The presence of ions and dissolved solids in a solution
104) A patient has a measured osmolality of 283 mOsm/kg and a calculated osmolality of 275 mOsm/kg. Calculate the osmolal gap.
0.97 mOsm/kg
1.03 mOsm/kg
8 mOsm/kg
556 mOsm/kg
105) Which condition is most consistent with the results below?
Test
Result
Osmolality
290 mmol/kg
Sodium
125 mmol/L
Total cholesterol
35.7 mmol/L
Cushing's syndrome
Hyperaldosteronism
Hypothyroidism
Pseudohyponatremia
106) In which disease is haemoglobin A2 elevated?
Alpha-thalassemia
Alport Syndrome
Beta-thalassemia
Hemolytic anemia
107) In spectrophotometry, what does wavelength refer to?
The distance between wave peaks
The number of waves per unit of time
The time taken for one full wave cycle
The vertical height of a wave
108) What is the purpose of a diffraction grating in a spectrophotometer?
To amplify radiant energy
To convert light into an electrical signal
To measure scattered light
To separate light into specific wavelengths
109) What is the formula for calculating the percent transmittance of a solution from its absorbance (A)?
Percent transmittance = 10(2 − A)
Percent transmittance = 10(A + 100)
Percent transmittance = 10(A × 100)
Percent transmittance = 10−A
110) What does the Sampson equation estimate the level of?
HDL cholesterol
LDL cholesterol
Lipoproteins
Total cholesterol
111) Which technique measures the amount of light absorbed by a solution at specific wavelengths?
Densitometry
Nephelometry
Refractometry
Spectrophotometry
112) At what pH are barbital buffers used in the serum protein electrophoresis test?
5.0
5.6
6.5
8.6
113) Which analytical technique separates components based on their affinity for a stationary phase and a mobile phase?
Chromatography
Nephelometry
Refractometry
Spectrophotometry
114) Given the results below, what is the osmolal gap?
Test
Result
Sodium
140 mmol/L
Glucose
5 mmol/L
BUN
10 mmol/L
Measured osmolality
301 mmol/kg
1 mOsm/kg
3 mOsm/kg
6 mOsm/kg
9 mOsm/kg
115) A 40-year-old man weighing 80 kg has a serum creatinine level of 106.3 µmol/L. Using the Cockcroft-Gault formula, calculate the creatinine clearance in mL/min.
32.8 mL/min
68.4 mL/min
92.6 mL/min
126.2 mL/min
116) In which of these situations should you discard a urine sample?
When the sample has no label
When the sample was collected one hour ago
When the sample was refrigerated overnight
When the urine appears cloudy
117) Which of these biochemistry results is critical?
ALT of 12 U/L in a pregnant woman during her third trimester
Creatinine of 707 µmol/L in a 13-year-old
Potassium of 4.2 mmol/L in a pre-dialysis patient
Total bilirubin of 15.4 µmol/L in a 1-day-old newborn
118) How does nephelometry differ from turbidimetry?
Nephelometry is used for concentrated solutions, while turbidimetry is used for dilute solutions
Nephelometry measures light at a 180° angle, whereas turbidimetry measures light at a 90° angle
Nephelometry measures scattered light while turbidimetry measures transmitted light
Nephelometry measures solutes, while turbidimetry measures solvents
119) Which type of acidosis best explains the arterial blood gas results below?
Test
Result
pH
7.39
PaCO₂
59 mmHg
Bicarbonate
39 mmol/L
Compensated metabolic acidosis
Compensated respiratory acidosis
Uncompensated metabolic acidosis
Uncompensated respiratory acidosis
120) What is the Henderson-Hasselbalch equation?
ph = pKa + log(conjugate base/acid)
PAO₂ = PiO₂ − PaCO₂/R
O₂ saturation = Hb × 1.34 + pO₂ × 0.003
pCO₂ = 1.5 [HCO₃] + 8
121) A high blood pH and a low PaCO₂ suggest which condition?
Hyperventilation
Hypoventilation
Increased bicarbonate absorption
Decreased bicarbonate absorption
122) A patient's blood gas results are as follows.
Test
Result
pH
7.26
PaCO₂
55 mmHg
What would these results be classified as?
Metabolic acidosis
Metabolic alkalosis
Respiratory acidosis
Respiratory alkalosis
123) A nurse collects an arterial blood specimen for blood gas analysis at 8:30 AM, but does not deliver it to the laboratory until 11 AM. What should the laboratory do?
Perform the test ASAP
Perform the test only if the specimen was submitted in ice water
Request a new sample
Request a venous blood specimen
124) Which electrode measures the partial pressure of oxygen in blood gas analysers?
Clark
Glass
Sanz
Severinghaus
125) According to the Beer–Lambert law, absorbance is directly proportional to:
the concentration of the solution
the temperature of the solution
the transmittance of the light
the wavelength of the incident light
126) Why is it important to clean sample probes on chemistry analysers routinely?
To ensure the cuvettes reach the correct temperature
To improve reagent shelf life
To prevent sample carryover
To prevent wear on the internal light source
127) Which technology do most modern analysers use to measure electrolyte concentrations?
Flame emission photometry
Gas chromatography
Ion-selective electrodes
Spectrophotometry
128) In chromatography, what is the term for the time it takes for a specific compound to pass through the system?
Elution time
Migration time
Pass time
Retention time
129) An anemic patient has the following blood test results.
Test
Result
RBC
3.6 × 10¹² cells/L
WBC
6.7×10⁹ cells/L
Hct
15%
Hgb
112 g/L
Reticulocyte percentage
9%
Given a maturation correction of 2.5 and assuming a normal hematocrit of 45%, calculate the reticulocyte production index.
1.2%
1.5%
1.8%
2.1%
130) Which white blood cell is most likely to be elevated in acute appendicitis?
Eosinophils
Lymphocytes
Monocytes
Neutrophils
131) Calculate the corrected reticulocyte percentage from the following results.
Test
Result
RBC (cells/L)
4.7×10¹²
WBC (cells/L)
4.5×10⁹
PLT (cells/L)
310×10⁹
Hct
30% (normal Hct = 45%)
Reticulocyte count
1.5%
0.5%
1%
1.5%
2%
132) A blood sample for a complete blood count shows red cell agglutination. What should you do?
Add EDTA
Dilute the sample with saline
Discard the sample
Warm the sample to 37°C
133) Blood test results from an automated analyser show a low red blood cell count, a low hematocrit, and a high MCV, MCH, and MCHC. Under a microscope, agglutination of red blood cells is observed. What step should be taken next?
Add kanamycin to the specimen and rerun the analysis
Dilute the specimen with isotonic diluent by a factor of 1:2 and rerun the analysis
Incubate the blood specimen at 37°C for 10–15 minutes and rerun the analysis
Mix the blood by vortex at the highest setting for 1–2 minutes and rerun the analysis
134) Which test result is falsely increased by lipemia during spectrophotometric analysis?
Haemoglobin
Hematocrit
Red blood cell count
White blood cell count
135) Which principle do hematology analysers use for counting red blood cells?
Campbell
Coulter
Stewart
Thompson
136) Below are normal and abnormal serum protein electrophoresis patterns:Which disease does the abnormal serum protein electrophoresis pattern indicate?
Hepatic cirrhosis
Hypogammaglobulinemia
Monoclonal gammopathy
Nephrotic syndrome
137) Leukocyte alkaline phosphatase (LAP) staining was performed on a blood specimen. The specimen was scored as follows.
Score
Neutrophils
0
40
+1
40
+2
15
+3
5
+4
0
Calculate the LAP score.
25
45
65
85
138) Which of these specimens would most likely be rejected for testing?
A hemolyzed potassium specimen
A nonfasting glucose specimen
A serum-separator tube that is only half-filled
An icteric bilirubin specimen
139) Leukocyte alkaline phosphatase (LAP) staining was performed on a blood specimen. The specimen was scored as follows.
Score
Neutrophils
0
10
+1
40
+2
30
+3
10
+4
10
Which condition most likely explains the result?
Chronic myeloid leukemia
Leukemoid reaction
Leukocytosis
Leukopenia
140) A patient has a potassium result of 7.2 mEq/L. Before reporting the result, what should the lab technician do?
Check the age of the patient
Check the serum for bacterial contamination
Check the serum for hemolysis
Test the serum for ketones
141) Which disorder correlates with these findings?
Test
Result
PLT
35 × 10⁹/L
HGB
72 g/L
INR
1.5
D-Dimer
22 mg/L
Acquired B
Disseminated intravascular coagulation
Folate deficiency anemia
Vitamin B12 deficiency anemia
142) Where should used microscope slides be disposed of?
Biological waste bin
Clinical waste bin
General waste bin
Sharps container
143) A doctor orders a blood smear for malaria. After collecting an EDTA blood specimen from the patient, the lab technician should prepare the blood smear within:
10 minutes
1 hour
6 hours
12 hours
144) Which of these would make a blood smear too thin?
A small drop size
Holding the spreader at a high angle
Spreading the blood too slowly
Using transfused blood
145) A man has the following blood test results.
Test
Result
Red cell count
5.4 × 10¹²/L
White cell count
6.5 × 10⁹/L
Platelets
660 × 10⁹/L
Which term applies to these results?
Anemia
Leukocytosis
Polycythemia
Thrombocytosis
146) A woman has the following blood test results.
Test
Result
Red cell count
7.8 × 10¹²/L
White cell count
7.5 × 10⁹/L
Platelets
242 × 10⁹/L
What term applies to these results?
Leukocytosis
Leukopenia
Polycythemia
Thrombocytopenia
147) The result of a patient's partial thromboplastin time (PTT) test is >300 seconds. Which of these could be the cause?
The patient had a high-fat meal before the blood draw
The patient has a vitamin K deficiency
The sample was contaminated with heparin
The sample was shaken vigorously
148) Which factor deficiency correlates with the following results?
Test
Result
PT
22 seconds
APTT
79 seconds
Fibrinogen
0.5 g/L
Factor I
Factor II
Factor V
Factor VII
149) In the prothrombin time test, what is the patient's plasma mixed with?
Calcium and activator
Calcium and tissue factor
Platelet lipids and prothrombin
Platelet lipids and thrombin
150) A patient has a prothrombin time of 12 seconds and an activated partial thromboplastin time of 25 seconds. Which condition does this indicate?
Antiphospholipid antibody syndrome
Factor V Leiden
Liver disease
Normal health
151) Which test is used to monitor warfarin therapy?
Erythrocyte sedimentation rate
Haemoglobin
International normalised ratio
Partial thromboplastin time
152) Which of these coagulation results requires immediate attention?
A PT of 10 seconds
A PT of 13 seconds in a patient on anticoagulants
An INR of 1.0
An INR of 4.5 for a patient on anticoagulants
153) A patient has a normal PT but an abnormal aPTT. A deficiency of which factor could be the cause?
Factor I
Factor II
Factor V
Factor VIII
154) Which autosomal dominant trait do these findings suggest?
Test
Result
Bleeding time
Prolonged
Platelet adhesiveness
Abnormal
PT
Normal
APTT
Normal
CBC
Normal
Factor X deficiency
Factor XI deficiency
Hemophilia A
Von Willebrand disease
155) Which of the following patients has microcytic red blood cells?
HCT
RBC (×10¹²/L)
Patient 1
42%
3.9
Patient 2
43%
5.5
Patient 3
50%
4.5
Patient 4
40%
3.1
Patient 1
Patient 2
Patient 3
Patient 4
156) Which red blood cell morphology is associated with iron deficiency anemia?
Macrocytic hypochromic
Macrocytic normochromic
Microcytic hypochromic
Normocytic normochromic
157) In healthy individuals, the hematocrit is roughly equal to three times the:
haemoglobin
mean corpuscular volume
red blood cell count
white blood cell count
158) How is mean corpuscular volume (MCV) calculated?
MCV = (HCT / RBC) × 10
MCV = (HGB / HCT) × 10
MCV = HGB / RBC
MCV = RBC/10 × HGB
159) A patient has the following hematology results.
Test
Result
RBC
4.0×10¹²/L
Haemoglobin
120 g/L
Hematocrit
0.45
Calculate the mean corpuscular haemoglobin (MCH).
12 pg
20 pg
30 pg
56 pg
160) A patient has the following hematology results.
Test
Result
RBC
4.2 × 10¹²/L
Haemoglobin
120 g/L
Hematocrit
0.4
Use the results to calculate the mean corpuscular haemoglobin concentration (MCHC).
150 g/L
200 g/L
250 g/L
300 g/L
161) Which red blood cell index is the average amount of haemoglobin in a single red blood cell?
RDW
MCV
MCH
RBC
162) Which of these complete blood count values is calculated, not measured?
Haemoglobin
MCHC
Platelet count
Red blood cell count
163) Which technique measures the physical and chemical properties of cells by analysing their light scattering and fluorescence emission?
Flow cytometry
Impedance counting
Osmometry
Refractometry
164) Which of these may cause a falsely elevated WBC count on automated analysers?
Cold agglutinins
Low hematocrit
Nucleated red blood cells
Platelet satellitism
165) Which microscope objective is used first when examining a stained blood film?
10x
40x
50x
70x
166) Leptocytes are found on a blood smear. Which of the following diseases is the LEAST likely?
Alpha-thalassemia
Haemoglobin C disease
Iron deficiency anemia
Spherocytosis
167) Which abnormal red blood cells are in the blood smear below?
Acanthocytes
Dacrocytes
Echinocytes
Schistocytes
168) Which type of red blood cell is the arrow pointing to in the blood smear below?
Burr
Sickle
Spur
Target
169) Which abnormal red blood cells are in the blood smear below?
Burr
Sickle
Spur
Target
170) A patient has a decreased factor VIII assay, a normal factor IX assay, and a prolonged aPTT. Which condition does the patient have?
Acute lymphoblastic leukemia
Acute myeloid leukaemia
Hemophilia A
Hemophilia B
171) Which condition best fits these test results?
Test
Result
Iron
85 µmol/L
Transferrin saturation
70%
Ferritin
875 µg/L
Anemia of chronic disease
Hemochromatosis
Iron deficiency anemia
Metabolic syndrome
172) Which of these hematology results is critical?
Haemoglobin: 50 g/L
INR: 2.9
Neutrophils: 7 × 10⁹/L
Platelets: 320 × 10⁹/L
173) A patient has symptoms of fatigue, weakness, and difficulty breathing. Blood tests reveal high iron, high ferritin, and a normal total iron binding capacity (TIBC). A peripheral blood smear shows red blood cells that are microcytic and hypochromic and have basophilic stippling. Pappenheimer bodies are also observed inside the red blood cells. What is the most likely diagnosis?
Acute intermittent porphyria
Porphyria cutanea tarda
Sickle cell anemia
Sideroblastic anemia
174) A patient has the following test results.
Test
Result
RBC
2.8 × 10¹²/L
Platelet count
75.0 × 10⁹/L
Janus kinase 2 (JAK2) mutation
Positive
Peripheral blood smear examination reveals dacryocytes and nucleated red blood cells. Which condition best fits these results?
Acute lymphoblastic leukemia
Chronic lymphocytic leukemia
Polycythemia vera
Primary myelofibrosis
175) What are target cells also known as?
Codocytes
Dacrocytes
Echinocytes
Spherocytes
176) What formula calculates red blood cell distribution width (RDW)?
RDW = (1 SD of the MCV / MCV) × 100
RDW = (HCT / RBC) × 10
RDW = (RBC × MCV) / 10
RDW = HGB / RBC
177) What is the main purpose of incident reports?
To assign legal responsibility for failures
To identify the individual responsible for the error
To improve safety and quality
To reduce the documentation workload
178) What does the Y-axis represent in a Bland-Altman plot?
The average of the two measurements
The dependent variable
The difference between the two measurements
The percentage error of the measurements
179) What does an internal audit evaluate?
Compliance with laboratory policies
Patient clinical outcomes
Patient satisfaction
The performance of other laboratories
180) Which of these is a pre-analytical error?
Calculating test results incorrectly
Interpreting results incorrectly
Mislabelling a blood specimen
Reporting a result on the wrong patient
181) What is the term for when readings become consistently lower or higher over time?
Calibration
Drift
Offset error
Unpredictability
182) Which of these is an example of a zero error?
A blood sample missing a label
A lab technician forgetting a zero when performing a calculation
A thermometer registering 102°C in boiling water instead of 100°C
Scales that give a reading of 1.1 g when nothing is placed on them
183) Random error mainly affects:
accuracy
external validity
internal validity
precision
184) Rapid antigen tests are typically described as:
qualitative and diagnostic
qualitative and screening
quantitative and diagnostic
quantitative and monitoring
185) You mistype a value into the LIS, causing a critical result to go unnoticed. Which action best demonstrates reflective practice?
Avoiding entering high-risk data in the future
Practising using the computer to prevent future mistakes
Requesting disciplinary action from your supervisor
Reviewing why the error happened
186) A coworker suggests skipping thermometer calibration because “the thermometer is always accurate.” What is the best response?
Agree to save time
Calibrate only if temperatures look wrong
Explain that calibration is essential for valid results
Stop using the thermometer entirely
187) The monitoring of laboratory processes to ensure the accuracy of test results is an aspect of:
corporate compliance
critical thinking
quality assurance
risk management
188) A hospital introduces a new blood glucose meter. The laboratory should:
create a survey for patient feedback
order new disposable gloves
revise the daily lab cleaning schedule
update the quality control procedures
189) During daily quality control for a prothrombin time test, the results fall consistently three standard deviations above the mean. After repeating the test using new reagents and fresh quality control material, the results remain three standard deviations above the mean. What should be the next course of action?
Check the coagulation analyser
Perform patient testing and multiply all results by 3
Perform patient testing on STAT samples only
Reduce the number of quality control runs to minimise variability
190) A lab technician runs controls for the L-pyrrolidonyl-β-naphthylamide (PYR) test. The results are as follows.
Organism
Result
Streptococcus pyogenes
Positive
Streptococcus agalactiae
Negative
Enterococcus faecalis
Positive
What action should the lab technician take?
Discard the reagent
Replace the dye
Retest the control organisms
Start testing patient samples since the test is functioning correctly
191) What is the term for a substance with an exact known value that can produce a solution of an exact concentration?
Benchmark
Control
Model
Standard
192) Which two organisms are used for quality control in the novobiocin susceptibility test?
Enterococcus faecalis and Enterococcus faecium
Escherichia coli and Enterococcus faecalis
Staphylococcus epidermidis and Staphylococcus saprophyticus
Streptococcus pyogenes and Streptococcus agalactiae
193) If a potassium calibration standard is too concentrated, patient results would be:
falsely high
falsely low
less precise
more precise
194) Which is an example of external quality control?
Daily glucose control testing
Instrument calibration
Levey-Jennings charts
Proficiency testing
195) What is the most likely cause of pseudohyperkalemia in patients with leukocytosis?
Contamination with a gadolinium MRI contrast agent
Interference from a blood pressure medication such as furosemide
Low level of magnesium in the blood
Rupture of blood cells in the blood sample
196) What is the purpose of proficiency testing?
To comply with environmental regulations
To evaluate a laboratory's testing accuracy
To monitor the rate of equipment malfunctions
To track the inventory levels of laboratory reagents
197) Which term means the ability of a procedure to detect only a substance of interest instead of other substances?
Analytical sensitivity
Analytical specificity
Diagnostic sensitivity
Diagnostic specificity
198) Checking and adjusting a measuring instrument against a known standard is called:
calibration
estimation
graduation
mensuration
199) Which of these best meets the definition of proficiency testing?
Inspections to prevent work-related accidents
Testing for illegal drugs in a biological sample
Tests to ensure workers have the skills and knowledge to perform their jobs
The testing of unknown samples sent to the laboratory
200) Where would you find the instructions for running quality controls for a specific assay?
Laboratory quality manual
Safety data sheet
Safety manual
Standard operating procedure
201) Which of the following is an example of a quality assurance indicator?
C-reactive protein test
Fume hoods
Laboratory turnaround time
Thorough records of exposure incidents
202) A substance with a known value that is used to ensure the validity and accuracy of test results is called a:
control
reagent
reference
sample
203) Which of the following quality control results is acceptable for the L-pyrrolidonyl-β-naphthylamide (PYR) test?
Enterococcus faecalis: positive
Escherichia coli: positive
Streptococcus agalactiae: positive
Streptococcus pyogenes: negative
204) In a nitrate reduction test, the broth remains colourless after nitrate A and B reagents are added, and still remains colourless after zinc is added. What does this indicate?
Nitrate is still present in the broth
Nitrate was reduced beyond nitrite, such as to nitrogen gas
Nitrate was reduced to nitrite
The reagents are invalid
205) The following chart compares an old method of performing a hematologic test (X-axis) with a new method (Y-axis). The squares represent samples tested with both methods and the black line is the line of best fit.What kind of bias does the new method show?
Constant
Non-linear
Proportional
Sampling
206) Which of these is a pre-analytical quality indicator?
Number of improperly labelled samples
Number of laboratory reporting errors
Percentage of results delivered late
Turnaround time
207) What kind of chart is a graphical presentation of run-to-run or day-to-day quality control values?
Eadie-Hofstee
Hanes-Woolf
Levey-Jennings
Lineweaver-Burk
208) Which ISO standard specifies requirements for quality and competence in medical laboratories?
ISO 15106
ISO 15189
ISO 15503
ISO 15513
209) If the same thing is measured many times and the measurements are always the same, this means the measurements are:
accurate
reliable
valid
variable
210) A machine consistently reports results that are lower than the true value. This is a problem with:
accuracy
precision
sensitivity
specificity
211) If two methods have a correlation coefficient of 0.98, this means they produce results that are:
completely different
identical
inaccurate
very similar
212) What does the coefficient of variation measure?
Accuracy
Precision
Sensitivity
Specificity
213) In a control run, two consecutive control results exceed the +2 SD limit. What does this indicate?
Contamination of control material
Random error due to poor precision
Systematic error due to poor accuracy
The control is normal, begin patient testing
214) What is precision?
The closeness of a result to the first standard deviation
The closeness of a result to the median value
The closeness of a result to the true value
The repeatability of a procedure
215) The following quality control results were obtained:
Test number
Result
1
13.1
2
13.0
3
13.1
4
13.1
5
13.0
Given that the target value for the control is 6.0 mmol/L ± 0.5 mmol/L, these results are:
precise but not accurate
accurate but not precise
both accurate and precise
neither accurate nor precise
216) Which term means the extent to which measurements agree with the true value?
Accuracy
Precision
Reliability
Reproducibility
217) What is the formula for calculating diagnostic sensitivity?
218) The following data pertains to a new diagnostic test.
True positives
30
True negatives
40
False positives
12
False negatives
10
Calculate the negative predictive value of the test.
80%
82%
90%
92%
219) A cancer screening test has the following results.
True positives
100
True negatives
15
False positives
85
False negatives
0
The test shows high:
accuracy
reproducibility
sensitivity
specificity
220) A diabetes screening test incorrectly detects diabetes in 5% of healthy patients who do not have diabetes. This means the:
sensitivity is 5%
sensitivity is 95%
specificity is 5%
specificity is 95%
221) A test suitable for screening purposes should have:
high sensitivity
high specificity
low sensitivity
low specificity
222) A lab assistant notices multiple near-expired Vacutainer tubes in the stock cupboard. What is the best thing to do?
Mix them randomly with the new tubes
Put the older tubes at the front
Return the tubes to the manufacturer for a refund
Throw them away
223) A courier delivery of specimen tubes is delayed, and your stock is running low. What is the best course of action?
Ask patients to bring tubes from home
Cancel all draws
Only collect STAT specimens
Reuse old tubes after autoclaving them
224) What is the term for the process of reducing the impact or likelihood of risks?
Risk implementation
Risk improvement
Risk mitigation
Risk retention
225) What is the term for a specimen with a known value that is used during calibration?
Compound
Control
Reagent
Standard
226) What is the purpose of instrument calibration?
To ensure the accuracy of the instrument
To ensure the instrument is properly cleaned
To prolong the battery life of instruments
To repair broken instruments
227) What is the term for when a control chart shows seven consecutive points that steadily increase in value?
Cycle
Outlier
Shift
Trend
228) Which Westgard rule is violated if two measurements in the same run have a 4 standard deviation difference?
1₂ₛ
2₂ₛ
4₁ₛ
R₄ₛ
229) A lab technician performs a control run and finds that four consecutive measurements exceed 1 standard deviation on the same side of the mean. What should the lab technician do?
Accept the control run
Accept the control run but make a note of the incident in the log file
Reject the control run and take corrective action
Rerun the control run
230) Below is a Levey-Jennings quality control chart representing the control values for 20 consecutive analyses:Which Westgard rule is violated in this chart?
10ₓ
1₂ₛ
4₁ₛ
R₄ₛ
231) In a control run for a serum magnesium test, a control exceeds two standard deviations above the mean and another control exceeds two standard deviations below the mean. What does this indicate?
Contamination of control material
Random error due to poor precision
Shift in the mean of the distribution
Systematic error due to poor accuracy
232) In a run of twenty control samples, a single control value falls two standard deviations outside of the mean. The other control readings are within the normal range, and no source of error can be identified. What is the most appropriate action to take?
Inform relevant clinicians that past results may be incorrect
Prepare a new reagent
Replace the equipment
Report patient results
233) The quality control chart of an instrument shows ten consecutive data points on one side of the mean. What should you do?
Accept the run
Reject the run and follow the method's troubleshooting procedures
Repeat the control sera on the next run
Report the results with a disclaimer that they may be erroneous
234) What are Levey-Jennings charts used for?
Determining how well a laboratory test is working
Logging the daily calibration of laboratory instruments
Summarising monthly performance statistics
Tracking the expiration dates of test reagents
235) What is the term for when a control chart shows seven consecutive points on the same side of the mean?
Cycle
Outlier
Shift
Trend
236) Which pathophysiological effect may result from transfusions of red blood cells with low 2,3-DPG levels?
Decreased carbon dioxide levels
Increased mixed venous oxygen tension
Lower blood viscosity
Tissue hypoxia
237) Which of these blood products needs to be irradiated before being transfused into patients?
Cryoprecipitate
Fresh frozen plasma
Frozen washed red cells
Granulocytes
238) Which of these conditions can the direct antiglobulin test detect?
Hemolytic disease of the fetus and newborn
Iron deficiency anemia
Myeloma
Sickle cell anemia
239) A 35-year-old woman needs three units of red blood cells. The laboratory performs an antibody screen to identify any blood group antibodies. The antibody screen is positive. According to the results of the antibody panel below, which antibody most likely caused the positive result?
Anti-C
Anti-D
Anti-E
Anti-K
240) What is the second-most important blood group system for blood transfusions?
Lutheran
MNS
P
Rh
241) What does reverse blood typing determine?
The antibodies in the patient's plasma
The antigens in the patient's plasma
The antigens on the patient's red blood cells
The presence of abnormal red blood cells
242) What cells are used to detect ABO antibodies in reverse blood grouping?
Dendritic cells
Erythroblasts
Mast cells
Red blood cells
243) For how long can donated platelets be stored at room temperature?
1 hour
4 hours
24 hours
5 days
244) What is the 4-hour rule in blood banking?
Blood donors should not be kept waiting in the waiting room for more than 4 hours
Blood donors should refrain from exercise for four hours after donating blood
Red blood cell units left out of refrigeration for more than 4 hours cannot be returned to the refrigerator
Transfusions of red blood cell units should be completed within 4 hours of their removal from refrigeration
245) What is the 30-minute rule in blood banking?
After a transfusion, the donor should sit in a quiet room for 30 minutes before going home
Red blood cell units left out of a refrigerator for more than 30 minutes cannot be returned to the refrigerator
The tourniquet should not be left on the patient's arm for more than 30 minutes
Transfusions of red blood cell units should take no longer than 30 minutes
246) Which condition automatically disqualifies stored blood for transfusion?
Icterus
Lipemia
Presence of clots
Refrigeration
247) What is seen microscopically in a positive Kleihauer-Betke test?
Blue maternal red blood cells and pale fetal red blood cells
Granular maternal red blood cells and blue fetal red blood cells
Pale maternal red blood cells and pink fetal red blood cells
Pink maternal red blood cells and granular fetal red blood cells
248) If a Kleihauer-Betke test shows 1.2% of fetal cells, how many 300 µg vials of RhIG would the mother need?
1
2
3
4
249) A Kleihauer-Betke test reports 2.2% fetal cells. What is the estimated volume of fetal blood in the maternal circulation?
110 mL
160 mL
220 mL
270 mL
250) What does an immediate spin crossmatch detect?
ABO incompatibility
Clinically significant IgG antibodies
Fetomaternal hemorrhage
Rh antibodies
251) A patient’s red blood cells react 1+ with anti-D, then react 3+ after AHG is added. What is the most likely RhD type?
Autoimmune hemolytic anemia
Hemolytic disease of the newborn
Hereditary spherocytosis
Weak D
252) A patient with Rh genotype DCe/DCe receives red blood cells from a dce/dce donor. Which alloantibody is the patient most likely to produce in response to the donor's red blood cells?
Anti-C
Anti-D
Anti-c
Anti-e
253) What blood type should a weak D–positive donor be reported as?
ABO incompatible
Rh positive
Rh negative
Inconclusive
254) What is the term for people whose cells have the D antigen?
D carriers
IgD-positive
Pro-D
Rh-positive
255) A patient's blood type testing results are as follows.
Red cell grouping
Serum grouping
anti-A
anti-B
anti-D
A₁ cells
B cells
0
4+
0
4+
0
What is the patient's blood group?
A+
A−
B+
B−
256) A person who is blood group B has:
A antigens and anti-A antibodies
A antigens and anti-B antibodies
B antigens and anti-A antibodies
B antigens and anti-B antibodies
257) A patient's ABO testing results are as follows.
Red cell grouping
Serum grouping
anti-A
anti-B
anti-A₁
A₁ cells
B cells
4+
0
0
2+
4+
What is the patient's ABO subtype?
A₁
A₂
A₁B
A₂B
258) A patient types as group A but shows agglutination with anti-A₁ lectin. What is the most likely explanation?
The patient is AB
The patient is A₁
The patient is A₂
The patient is weak D
259) A patient's ABO testing results are as follows.
Red cell grouping
Serum grouping
anti-A
anti-B
A cells
B cells
4+
1+
0
4+
What is the likely cause of these results?
Acquired B
Chimerism
Hypogammaglobulinemia
Immunosuppression
260) In blood typing, what colour may anti-A be dyed as a quality control measure?
Blue
Green
Red
Yellow
261) A patient's ABO testing results are as follows.
Forward typing
Reverse typing
anti-A
anti-B
A cells
B cells
+
0
0
+
What is the patient's ABO type?
Group A
Group AB
Group B
Group O
262) Refer to the blood typing test results below.
anti-A
anti-B
anti-D
Rh type
+
0
+
DCe/dce
What is the patient's blood type?
A+
A−
B+
B−
263) Which type of autoantibody is most likely if the screening cells, auto control, and all panel cells react during the antihuman globulin phase of antibody screening?
Anti-D
Anti-K
Cold
Warm
264) Which of these medications can cause a false positive result on the direct antiglobulin test?
Aspirin
Clopidogrel
Levothyroxine
Penicillin
265) After adding IgG-coated red blood cells to a negative antiglobulin test, no agglutination occurs. What is the most appropriate conclusion?
The original negative test result is valid
The patient has antibodies against their own red blood cells
The saline washings were adequate
The test needs to be repeated
266) A technician has prepared IgG-coated check cells. To verify the check cells work properly, the technician mixes the check cells with anti-IgG. The check cells react 4+. What does this indicate?
A problem with the anti-IgG
A problem with the check cells
Centrifugation was too fast
Normal result
267) A blood donor is tested with Rh antisera. The results are as follows.
anti-D
anti-C
anti-E
anti-c
anti-e
+
+
0
+
+
What is the donor's most probable Rh genotype?
DCe/DCe
DCe/DcE
DCe/dce
Dce/dCe
268) Urine is applied to a pregnancy strip. After several minutes, the strip shows no lines. This means the result is:
inconclusive
invalid
negative
positive
269) Which statement about the arterial blood gas (ABG) test is true?
ABG samples should be analysed within 15 minutes of collection
ABG specimens are collected in a plain red top tube
ABG specimens must be frozen immediately after collection
ABG specimens must be tested within 24 hours
270) You receive a request for a test you are unfamiliar with. What is the safest next step?
Ask another MLA what they would do
Check the lab’s SOP
Perform every step twice
Refuse to perform the test
271) Which test can rule out a false positive result for bilirubin on a urine strip?
Acetest
Clinitest
Ictotest
Sulphosalicylic acid (SSA)
272) Urine samples for culture should be collected in containers that:
are narrow-mouthed
are sterile
contain an anticoagulant
have been cleaned with disinfectant
273) Which chemical is used to preserve urine samples for bacterial culture tests?
Ammonia
Boric acid
Calcium hydroxide
Potassium hydroxide
274) Which chemical does the urine glucose dipstick test use?
Glucose oxidase
Glucose oxide
Glucose reductase
Hexichloridine
275) Which is the preferred additive for collecting blood culture specimens?
Citrate phosphate dextrose
Potassium oxalate
Sodium heparin
Sodium polyanethol sulphonate
276) A courier delivers a chain of custody sample to a technician at a laboratory. Who needs to sign and date the chain of custody form?
Only the courier
Only the laboratory technician
The courier and the laboratory technician
The laboratory manager and the laboratory technician
277) Which of the following is NOT required on a chain of custody form?
The name of the patient's doctor
The name of the person who collected the specimen
The time the specimen was collected
The type of specimen collected
278) What is meant by a chain of custody?
Chains used to restrain a prisoner during a medical procedure
The hierarchy of caregivers who have custody of a child
The management hierarchy in a hospital
The process of documenting the handling of evidence
279) Why is it crucial to follow chain-of-custody procedures in forensic testing?
To ensure test results are confidential
To maintain the integrity of evidence
To prevent post-analytical errors
To verify the patient’s insurance information
280) You receive a specimen that should come with a chain of custody form. However, the form is missing. What should you do?
Ask the courier to complete a new form
Fill out a new form yourself
Reject the specimen
Test the specimen, but add a comment
281) Which of these tests requires chain of custody documentation?
Complete blood count
Fetal fibronectin
Free light chains test
Legal paternity test
282) You think a patient's name is John Smith but you are unsure. Which of these questions is the best way to confirm the patient's name?
"Are you John Smith?"
"I have to confirm your name. Can you confirm that your name is John Smith?"
"Is your name the same as the name written here on this form?"
"What is your name?"
283) What visual sign suggests a plasma sample is hemolyzed?
Air bubbles
Blood clots
Decreased transparency
Pink or cherry red colour
284) Why are blood samples for ammonia tests transported on ice?
To prevent the breakdown of ammonia
To prevent the conversion of ammonia to nitrite
To prevent the generation of ammonia
To prevent the growth of pathogenic microorganisms
285) A courier arrives early to pick up specimens, but some of the samples are still centrifuging. What should you do?
Ask the courier to wait
Skip centrifugation for all non-STAT tubes
Stop the centrifuge early
Tell the courier to come back tomorrow
286) Which of these specimens should be rejected for testing?
A blood glucose sample exposed to light
A frozen urine culture sample
A refrigerated blood sample for TSH testing
A urine creatinine sample transported at room temperature
287) A nurse insists a specimen must be transported on ice, but the lab manual says it must be transported at room temperature. What should you do?
Do what the nurse says
Follow the lab manual and document the reasoning
Refuse to transport the specimen
Transport half the specimen on ice and half at room temperature
288) A urine specimen without preservative arrives at the laboratory five hours after it was collected. To be acceptable for culture testing, the specimen must have been:
kept at room temperature
kept frozen
kept refrigerated
mixed with an antiglycolytic agent
289) A patient produces a semen sample at home. When bringing the sample to the clinic, the patient should keep the sample:
at room temperature
next to a hot water bottle
on ice
refrigerated
290) A cerebrospinal fluid (CSF) specimen is collected for culture, but the laboratory cannot process the specimen immediately. At what temperature should the specimen be maintained until testing?
Frozen at –60°C
Frozen at –20°C
Refrigerator temperature (2–8°C)
Room temperature
291) Which test result becomes falsely increased by removing the stopper from a blood specimen?
Cortisol
Glucose
pCO₂
pH
292) When a blood sample is left at room temperature, which of these analytes increases?
Carbon dioxide
Glucose
Haemoglobin
Potassium
293) Extra precautions are necessary when labelling specimens for:
C&S
blood typing
factor assays
therapeutic drug monitoring
294) Which class of dangerous goods includes infectious substances?
2
3
5
6
295) In Type P650 packaging, the primary and secondary receptacles must be:
dustproof
heatproof
leakproof
sunproof
296) What are diagnostic specimens suspected of containing Salmonella transported as?
Category A
Category B
Class 1
Class 2
297) Category B infectious substances must be shipped with a label that says:
UN 2814 – Infectious Substance, Category B, Affecting Humans
UN 3291 – Clinical Waste, Category B
UN 3373 – Biological Substance, Category B
UN 3549 – Medical Waste, Category B, Affecting Humans
298) Infectious substances that pose the highest risk of infection during transportation and are capable of causing permanent disability, life-threatening disease, or fatal disease are known as ______________ substances.
Category A
Category B
Category C
Type 1B
299) What packaging is used to ship Category A infectious substances?
A610
A630
P620
P650
300) Which of the following is an example of a qualitative test?
Blood gas test
Fecal occult blood test
Lipid panel
Serum glucose level
301) A urine dipstick reports glucose as “++”. This result is:
categorical
qualitative
quantitative
semi-quantitative
302) Which of these results is most likely to require notification to public health authorities?
Acute allergic reaction
Positive HIV test
Positive mononucleosis test
Positive pregnancy test
303) Which factor should be considered first when an add-on request is received?
The availability of the lab technician
The cost of the additional test
The original test result
The specimen's stability
304) Urine microscopic examination is performed on:
diluted urine
refrigerated urine
the sediment suspension of centrifuged urine
the supernatant of a centrifuged urine
305) A patient with fatigue and nausea has the following kidney function results.
Test
Result
Urine pH
5.5
Urine glucose
0 mmol/L
GFR
31
UACR
>6.9 mg/mmol
No blood or abnormal cells can be seen in the urine. Which of these diagnoses is most compatible with the findings?
Bladder cancer
Diabetes
Kidney disease
Prostate cancer
306) When preparing a urine specimen for microscopic analysis, which of these steps comes first?
Add one drop of stain to the tube
Centrifuge the urine sample
Place a coverslip over the sample
Transfer one drop of the sediment to a microscope slide
307) Which test confirms a positive immunoassay drug test?
Enzyme-linked immunosorbent assay
Gas chromatography-mass spectrometry
Ion-selective electrode
Thin-layer chromatography
308) A 40-year-old man weighing 70 kg has a serum creatinine level of 1.00 mg/dL. Using the Cockcroft-Gault equation, estimate the man's creatinine clearance in mL per minute.
97.2
102.7
107.3
112.9
309) A urine sample tested positive for urine protein by a dipstick test but negative by the sulphosalicylic acid method. What is the probable cause of these conflicting results?
The urine contained crystals
The urine contained ketones
The urine was very alkaline
The urine was very turbid
310) Which of these tests does NOT require a 24-hour urine specimen?
Catecholamines
Creatinine clearance
Ketones
Ketosteroids
311) The following results were obtained from the urine of a 31-year-old woman.
Parameter
Result
Colour
Pale yellow
Appearance
Cloudy
Specific gravity
1.015
pH
6.0
Glucose
Negative
Protein
1+
Bacteria
Many
WBC casts
Many
WBC/HPF
30
Which disease are these results most compatible with?
Glomerulonephritis
Pyelonephritis
Renal calculus
Vaginitis
312) The following results were obtained from a urine specimen.
Parameter
Result
White blood cells
Positive
White blood cell casts
Positive
Bacteria
Negative
Which condition best fits these results?
Diabetes mellitus
Interstitial nephritis
Nephrotic syndrome
Pyelonephritis
313) The following results were obtained from the urine of a 45-year-old male.
Parameter
Result
Colour
Pale yellow
Appearance
Foamy
Specific gravity
1.030
pH
6.5
Glucose
Negative
Protein
2+
Bacteria
Negative
WBC
Negative
Fatty casts
Many
Oval fat bodies
Many
What is the most likely diagnosis?
Acute pyelonephritis
Cystitis
Nephrotic syndrome
Polycystic kidney disease
314) How does GC-MS differ from immunoassay drug screening?
GC-MS has higher cross-reactivity
GC-MS is the initial screening test
GC-MS provides definitive confirmation
GC-MS uses antibody-antigen binding to detect drugs
315) The presence of epithelial cell casts in urine indicates:
liver disease
nephrotic syndrome
pyelonephritis
renal tubular damage
316) A patient's cerebrospinal fluid has a lactate value of 40 mg/dL. Which type of meningitis does this indicate?
Bacterial
Fungal
Tubercular
Viral
317) What is the normal adult range for leukocyte count?
0.5–7.0 × 10⁹/L
2.5–9.0 × 10⁹/L
4.5–11.0 × 10⁹/L
6.5–13.0 × 10⁹/L
318) Which of these patients has results consistent with fluid overload?
Patient
Gender
Creatinine
BUN
1
Male
131 µmol/L
11.8 mmol/L
2
Male
32 µmol/L
0.5 mmol/L
3
Female
95 µmol/L
3.4 mmol/L
4
Male
70 µmol/L
7.1 mmol/L
Patient 1
Patient 2
Patient 3
Patient 4
319) Which of these hematology tests is outside the normal range?
PLT: 257 × 10⁹/L
RBC: 7.2 × 10¹²/L
WBC: 5.4 × 10⁹/L
WBC: 8.1 × 10⁹/L
320) What is the expected result of a negative control of an immunohistochemical test?
No specific staining
Staining of all cells
Staining of cells expected to express the antigen
Staining of cells without the antigen
321) Which of the following is a critical value in an adult?
Cholesterol level of 6.5 mmol/L
Glucose level of 2.5 mmol/L
Haemoglobin A1c of 6.5%
Potassium level of 4.5 mmol/L
322) Microscopic examination of the urine of a patient with kidney stones reveals amber, barrel-shaped crystals. The pH of the urine is 5.5. What are these crystals?
Calcium carbonate
Calcium phosphate
Struvite
Uric acid
323) Incident reports should be:
filed with the associated visit
stored separately from the health record
the first document seen when accessing a record
thrown out once the incident is investigated
324) What ethical dilemma does a healthcare provider face when a patient refuses a life-saving treatment?
Being honest vs. withholding information
Healthcare needs vs. resource allocations
Patient autonomy vs. the duty to provide care
Pro-choice vs. pro-life
325) Which principle of data ethics emphasises that organisations collecting and using data are answerable for the consequences of their actions?
Accountability
Consent
Equity
Privacy protection
326) A researcher is given copies of patient records for a study. The researcher replaces the real names in the records with fake names to protect the privacy of the patients. He also keeps a record that links the real names to the fake names so he can re-identify the data if necessary. What type of information has the researcher created?
Aggregate
Anonymous
De-identified
Pseudonymized
327) What is the purpose of peer review in scientific research?
To decrease cognitive overload
To ensure research is accurate
To increase the publication rate
To reduce research costs
328) Which sources provide the highest level of evidence?
Case series and case reports
Case-control studies and case reports
Expert opinions and retrospective comparative studies
Systematic reviews and meta-analyses
329) In which study does neither the researcher nor the participants know to which group the participants belong?
Control group
Double blind
Placebo
Random sampling
330) What kind of study monitors people over ten years?
Concurrent
Cross-sectional
Longitudinal
Retrospective
331) Which technique identifies the underlying cause of problems?
Affinity diagram
Brainstorming
Proactive risk assessment
Root cause analysis
332) Which research method involves manipulating an independent variable to observe its effect on a dependent variable?
Experimental
Longitudinal
Mixed
Secondary data analysis
333) What statistical method draws conclusions about the larger population by examining a sample of that population?
Binomial
Descriptive
Inferential
Special
334) Which type of research method only uses numerical data?
Case study
Interview
Qualitative
Quantitative
335) What type of statistics provides simple summaries about the basic features of the data?
Descriptive
Hypothetical
Inferential
Predictive
336) Which type of study compares individuals with and without a particular disease or condition?
Case control
Cohort
Cross sectional
Randomised clinical trial
337) What is the main function of a literature review?
To design a research instrument
To develop a case study
To summarise current knowledge
To test a hypothesis
338) What is the name of a study where only the experimenter knows which group each participant is in, while the participants themselves are unaware?
Double-blind
Non-blind
Relational sampling
Single-blind
339) Which research method involves coding and categorising documents, interviews, or videos to identify themes, patterns, or trends within the data?
Case study
Content analysis
Cross-sectional study
Meta-analysis
340) Which of these is true about random sampling?
All subjects are unaware that there is an experiment taking place
All subjects have an equal chance of being selected
Subjects are chosen as they become available
Subjects are selected based on convenience
341) Which of these research methods collects qualitative data?
Closed-ended survey
Experiment
Interview
Rating scale
342) Which of the following is an example of unconscious bias in research?
Falsifying data to support a hypothesis
Failing to disclose funding sources
Noticing only data fitting theories
Randomising participants to different groups
343) A correlational analysis reveals a correlation of +0.95 between the length of hospital stay and the risk of acquiring a hospital-acquired infection. What does this tell you?
The length of hospital stay and the risk of acquiring a hospital-acquired infection increase together
The length of hospital stay and the risk of acquiring a hospital-acquired infection vary in opposite directions
The longer patients stay in the hospital, the more infections they will get
The more infections patients get, the longer they will have to stay in the hospital
344) Which type of study involves observations of a population at a single point in time?
Cohort
Cross-sectional
Longitudinal
Qualitative
345) What type of research would be used to study how age affects serum glucose level?
Correlational
Evaluation
Experimental
Retrospective
346) A scale that has "excellent", "very good", "good", "fair", and "poor" as variables is an example of which scale of measurement?
Interval
Nominal
Ordinal
Ratio
347) What is the name for a combination of results from several studies?
A meta-analysis
An experiment
Data mining
Epidemiology
348) An experiment tests the effects of a new antihypertensive medication on blood pressure. In this experiment, the medication is:
a confounding factor
the dependent variable
the hypothesis
the independent variable
349) Which of these helps maintain data integrity?
Calculating statistics manually
Increasing the frequency of data collection
Using WORM (write once, read many) storage
Using multiple sources of data
350) What is data stewardship?
The process of storing data in multiple locations
The regulation of the collection and access of data
The standardisation of data collection formats
The task of ensuring data quality and security
351) What internet-based platform lets people view their personal health information?
Patient portal
Telehealth
Virtual care
e-Health
352) Patients have the right to inspect and obtain a copy of their personal health information:
for as long as the PHI is maintained
for six years
for 1 year
forever
353) Which type of personal health information is used for direct patient care and has the strictest privacy regulations?
Aggregate
De-identified
Identifiable
Pseudonymized
354) What is the most effective type of hazard control in the ISO 45001 Hierarchy of Controls?
Administrative controls
Elimination
Engineering controls
Substitution
355) What is the least effective type of hazard control in the ISO 45001 Hierarchy of Controls?
Administrative controls
Engineering controls
Personal protective equipment
Substitution
356) The Globally Harmonised System of Classification and Labelling of Chemicals has two signal words for hazardous chemicals: "Danger" and:
Attention
Caution
Notice
Warning
357) Which worldwide system gives recommendations on how to label hazardous chemicals?
GHS
HCS
HPR
HazCom
358) A medical assistant prints out a patient's demographic information so the patient can review it for errors. Once the patient has reviewed the information, what should the medical assistant do with it?
Dispose of it by shredding
Give it to the doctor
Give it to the patient to take home
Put it in the patient’s file
359) In flow cytometry, what is the term for digitally isolating a specific sub-population of cells for further analysis?
Binning
Compensation
Gating
Partitioning
360) Which instrument analyses cells by passing them one by one through a laser beam?
Counting chamber
Electrophoresis chamber
Flow cytometer
Spectrophotometer
361) What temperature is the incubation phase of the indirect antiglobulin test?
22°C
37°C
56°C
100°C
362) Which antigen system is NOT associated with hemolytic disease of the fetus and newborn?
ABO
Duffy
Lewis
RhD
363) Which type of antibody causes false positives in immunoassay testing due to its ability to bind to different components used in these tests?
Anti-nuclear
Heterophile
IgE
Monoclonal
364) If a polyspecific direct antiglobulin test (DAT) is positive, what test would be used next to investigate the possibility of cold agglutinin disease?
Indirect antiglobulin test
Monospecific DAT for C3d and IgG
Reverse ABO typing
Von Willebrand factor assay
365) In flow cytometry, what does forward scatter indicate?
Cell complexity
Cell fluorescence
Cell granularity
Cell size
366) Which disease do these results suggest?
Test
Result
WBC
5,200 cells per µL
Lymphocytes
16%
CD4
7%
Addison disease
Graves' disease
HIV
Type 1 diabetes
367) The results of an antibody titer are below.
1:2
1:4
1:8
1:16
1:32
1:64
1:128
+
+
+
+
+
−
−
What is the antibody titer of these results?
1:2
1:32
1:64
1:128
368) In standard two-tier testing for Lyme disease, what test is used for the second step?
C-reactive protein (CRP) test
ELISA
Rapid antigen test
Western blot
369) Which transmission-based precaution is required for a patient infected with a multidrug-resistant organism?
Airborne
Contact
Droplet
Protective isolation
370) What does the Ōritetanga article of the Treaty of Waitangi address?
Equality
Racism
Representation
Self-determination
371) Which organisation accredits medical laboratories in New Zealand?
Health and Disability Commissioner
International Accreditation New Zealand (IANZ)
Medical Sciences Council of New Zealand
Ministry of Health
372) Which organisation regulates the registration and practice of medical laboratory scientists in New Zealand?
Health Quality & Safety Commission
Medical Sciences Council of New Zealand (MSCNZ)
Ministry of Health
New Zealand Institute of Medical Laboratory Science (NZIMLS)
373) Which act requires health practitioners and laboratories to report cases of notifiable diseases to a Medical Officer of Health?
Health Act 1956
Health and Disability Services (Safety) Act 2001
Health and Safety at Work Act 2015
Public Health and Disability Act 2000
374) Under the MSCNZ CPD programme, what is the minimum number of hours of CPD health practitioners must achieve each two-year period?
120 hours
150 hours
40 hours
80 hours
375) Which New Zealand law governs patient privacy and the protection of personal health information?
Health Practitioners Competence Assurance Act 2003
Health and Safety at Work Act 2015
Medicines Act 1981
Privacy Act 2020
376) Which agency administers how dangerous goods can be safely transported by land?
Environmental Protection Authority
Ministry of Business, Innovation and Employment (MBIE)
NZ Transport Agency
New Zealand Police
377) What does the “Protection” principle in the Treaty of Waitangi protect?
Healthcare workers
Minors
Māori rights
Protected personal information
378) Which act gives the Medical Sciences Council of New Zealand the authority to regulate medical laboratory scientists?
Accident Compensation Act 2001
Health Practitioners Competence Assurance Act 2003
Health and Disability Commissioner Act 1994
Medicines Act 1981
379) What is the professional association and main continuing education provider for medical laboratory scientists and technicians in New Zealand?
Health Quality & Safety Commission
Medical Sciences Council of New Zealand (MSCNZ)
New Zealand Institute of Medical Laboratory Science (NZIMLS)
Te Whatu Ora – Health New Zealand
380) Which Māori word is derived from a word meaning "right" or "correct" and refers to the Māori customary system of values, practices, and protocols?
Kaitiakitanga
Manaakitanga
Tikanga
Whanaungatanga
381) A medical laboratory technician is returning to the workforce after a five-year break. Which law requires them to demonstrate that they can still perform their job?
Health Practitioners Competence Assurance Act 2003
Health and Disability Commissioner (Code of Consumer Rights)
Health and Safety at Work Act 2015
Privacy Act 2020
382) Which organisation investigates complaints about healthcare providers in New Zealand?
Health and Disability Commissioner
International Accreditation New Zealand
Medical Sciences Council of New Zealand
Ministry of Health
383) Which of these diseases is notifiable to a Medical Officer of Health?
Hepatitis A
Mononucleosis
Toxoplasmosis
Varicella
384) In spectrophotometry, what range is the visible spectrum?
12–18 nanometres
120–180 nanometres
200–400 nanometres
400–700 nanometres
385) For data distributed normally, how many values are within two standard deviations from the mean?
50%
68%
83%
95%
386) If the mean for an analyte is 10.1 mmol/L and the standard deviation is 1.7 mmol/L, calculate a control range that includes the values within two standard deviations of the mean.
2.3–4.7 mmol/L
6.7–13.5 mmol/L
8.4–11.8 mmol/L
9.8–10.5 mmol/L
387) What is the probability of three consecutive control values exceeding ±2 standard deviations by chance alone?
1:400
1:1,600
1:8,000
1:16,000
388) Which acceptable range do laboratories typically use for quality control results?
One standard deviation from the mean
Two standard deviations from the mean
The middle 68% of the results
The outer 5% of the results
389) If a data set has a mean of 7.2 and a standard deviation of 1.2, what range of values falls within two standard deviations of the mean?
4.8–9.6
6.0–8.4
7.2–9.6
8.6–10.2
390) If a control has a mean of 5 and a standard deviation of 1, what range should encompass 95% of the observed values?
1–9
2–8
3–7
4–6
391) How many of the following quality control results are beyond 2 standard deviations from the mean?
QC results
120, 289, 365, 432, 678
Mean
377
Coefficient of variation
15%
1
2
3
4
392) Automated white blood cell counts are performed on 29 blood samples. The mean value is 8.0 cells/nL, and the standard deviation is 1.2 cells/nL. What is the coefficient of variation?
15%
30%
45%
60%
393) A thyroid-stimulating hormone (TSH) quality control chart has the following data for the normal control.
Statistic
Value
Count
43
Mean
2.4 mIU/L
2 SD
1.2 mIU/L
Calculate the coefficient of variation for the control.
25%
35%
40%
50%
394) The following values were obtained from a quality control test: 0, 0, 2, 2, 2, 3, 4, 5, 5, 7. Calculate the mean of these values.
3
3.25
3.5
3.75
395) Which statistical test is used to compare the means of two groups?
Linear regression
Median
One-way ANOVA
Standard t-test
396) Which of these statistics is a measure of central tendency?
Coefficient of variation
Mean
Range
Standard deviation
397) Where should bulk quantities of acetone be kept?
Biological safety cabinet
Flammable storage cabinet
Freezer
Refrigerator
398) Where should stock ethanol solutions be stored?
Flammable safety cabinet
Freezer
Fume hood
Refrigerator
399) An acetone spill occurs on the bench near a lit Bunsen burner. What should be the first action?
Clean up the spill
Move everyone to fresh air
Turn off the flame
Wash eyes with water
400) Why must you never use a household vacuum cleaner to clean a mercury spill?
Mercury causes vacuums to ignite
Mercury corrodes vacuums
Mercury reacts with plastic to create chlorine gas
Vacuums spread mercury into the air
401) Which cleaning product releases a toxic gas when used to clean urine spills?
Chlorine bleach
Hydrogen peroxide
Isopropyl alcohol
Povidone-iodine
402) Mercury spills are most dangerous when the spill occurs:
in a fume hood
in a poorly ventilated area
near sources of ignition
near the flammable storage cabinet
403) Which of these dilutions is the most effective disinfectant?
10% ethyl alcohol
40% ethyl alcohol
70% ethyl alcohol
90% ethyl alcohol
404) A disinfectant may become ineffective if:
it is kept in a dark place
it is left at room temperature
it is not diluted properly
the container is kept tightly sealed during storage
405) Which of these items is NOT found in biohazard spill kits?
Autoclavable waste bags
Disinfectant
Petri dishes
Safety goggles
406) What is the first step when a biological spill occurs outside a biosafety cabinet?
Alert people nearby
Cover the spill with paper towels
Fill in an incident report form
Put on PPE
407) Which chemical is best for neutralising weak acid spills?
Acetic acid
Boric acid
Sodium bicarbonate
Sodium hydroxide
408) After cleaning a biological spill, where should you place the waste cleaning materials?
In a biohazard bag
In a biosafety cabinet
In a sharps container
In the regular garbage
409) What is the first step when cleaning up a blood spill?
Pour disinfectant onto the spill
Put on PPE
Record the incident in the incident book
Wash your hands
410) A laboratory has an autoclave rule stating "Do not fill containers more than half full with liquids". If a lab technician has to prepare 220 ml of media for autoclaving, which of these volume containers would comply with the rule?
100 mL
300 mL
400 mL
500 mL
411) Why must all air be removed from an autoclave before switching it on?
Air causes cold spots in the autoclave
Air increases the risk of explosion
Any water in the air will dilute the sterilizing agents
Steam can break the glassware
412) What is the greatest risk when opening an autoclave immediately after a cycle finishes?
Burns from hot steam
Electrical shock
Exposure to carbon monoxide
Radiation exposure
413) A lab assistant is wrapping an instrument for autoclaving but discovers the wrapper has a hole. What should the lab assistant do?
Get a new wrapper
Ignore the hole and continue wrapping the instrument
Repair the hole with adhesive tape
Throw the instrument away
414) Which of these is a Standard Precaution?
Fire extinguisher
Hand hygiene
Keeping chemical containers closed
Radiation shielding
415) A test gives the following control values.
Day
Result (U/L)
1
1.2
2
1.5
3
1.8
4
2.1
What do these values demonstrate?
A repeating pattern
A trend
An outlier
Random variation
416) If two variables have a positive relationship, it means:
they have a strong correlation
when one variable decreases, the other increases
when one variable increases, the other decreases
when one variable increases, the other increases
417) A one molal solution contains one mole of solute per ________ of solvent.
gram
kilogram
litre
mole
418) An automated cell counter gives a result of 6,500 cells/mm³. However, the doctor's clinic requires the result in cells/L. What result should you report to the doctor?
6.5 x 10⁶ cells/L
6.5 x 10⁷ cells/L
6.5 x 10⁸ cells/L
6.5 x 10⁹ cells/L
419) What is a bimodal dataset?
A dataset with only two data points
A dataset with two distinct peaks
A dataset with two means
A dataset with two medians
420) What is the following graph shape?
Normal distribution
Polynomial function
Quadratic chart
Reciprocal graph
421) What is a non-Gaussian distribution?
A distribution that is not a symmetric, bell-shaped curve
A distribution that only contains categorical data
A distribution where the mean, median, and mode are all equal
A distribution with a standard deviation less than zero
422) 1 L of a 0.1 M potassium hydroxide solution neutralises 50 mL of a sulphuric acid solution. What is the molarity of the sulphuric acid solution?
0.005 M
0.045 M
0.5 M
1 M
423) A patient has the following test results.
Test
Result
Sodium
140 mmol/L
Glucose
20 mmol/L
BUN
2 mmol/L
Using the Dorwart and Chalmers formula, calculate the patient's serum osmolality.
107.4 mmol/L
193.9 mmol/L
282.4 mmol/L
382.9 mmol/L
424) What is the osmolarity of a 1 mol/L NaCl solution?
1 Osm/L
2 Osm/L
3 Osm/L
4 Osm/L
425) A patient's serum iron test results are as follows.
Test
Result
Serum iron concentration
20 µmol/L
Total iron-binding capacity
80 µmol/L
Calculate the percentage transferrin saturation.
4%
5%
10%
25%
426) A patient has the following test results.
Test
Result
Total protein level
8.3 g/dL
Albumin level
3.4 g/dL
What is the patient's calculated globulin?
0.4 g/dL
2.4 g/dL
4.9 g/dL
11.7 g/dL
427) What type of fire extinguisher can be used on methane gas fires?
ABC powder
AFFF foam
Carbon dioxide
Water
428) A fire extinguisher has the following symbol:This fire extinguisher:
can extinguish electrical fires
cannot be used on electrical equipment
carries a risk of electric shock
requires charging before use
429) Which of these chemicals is flammable?
Acetone
Carbon tetrachloride
Chloroform
Saline
430) A fire extinguisher has the following symbol.This fire extinguisher:
can be used on fires involving flammable liquids or gases
requires annual top-ups of type B fuel
should be stored in a separate room from flammable chemicals
uses kerosene to extinguish fires
431) When handling strong acids in a laboratory:
add water to acid when diluting
wear eye protection
wear latex gloves
wear open-toed shoes
432) Dry ice is dangerous because it burns, suffocates and:
can explode
catches fire when exposed to air
causes viral infections
is highly toxic
433) Where should you handle chemicals that produce harmful vapours?
Autoclave
Biological safety cabinet
Fume hood
Sealed room
434) Which of these is a safety measure when working with xylene?
Store it in a refrigerator
Use a HEPA-filtered mask
Use it in a biological safety cabinet
Use it under a fume hood
435) In the context of occupational health and safety, what does STEL stand for?
Short-term exposure limit
Standard task exposure limit
Standard task equipment list
Short-term emergency lighting
436) What dangerous chemical is produced when bleach is mixed with acid?
arsenic trioxide
chlorine gas
phosgene
sodium cyanide
437) What is the main hazard of ethanol?
Carcinogenicity
Corrosiveness
Flammability
Toxicity
438) Why is mercury hazardous?
It forms an explosive precipitate on standing
It forms toxic vapours
It is flammable
It reacts violently with water
439) A lab assistant accidentally drinks a beaker of Zenker's fluid. He says he feels a burning sensation in his mouth and throat. What is the first action you should take?
Call emergency services
Give the lab assistant water to drink
Place the lab assistant in the recovery position
Take the lab assistant outside for fresh air
440) Which of these is a danger of liquid nitrogen?
It can catch fire
It can cause cancer
It can deplete oxygen in the air
It can explode
441) Which of these is written information that must be supplied by manufacturers of chemicals and hazardous materials?
OSM
SDS
TOSM
TSM
442) In which of these situations should a lab technician remove their gloves?
Before adjusting the focus knobs on a microscope
Before loading the autoclave
Before using a restroom
For cleaning a chemical spill
443) Which of these statements about nitrile gloves is true?
Nitrile gloves are a good substitute for latex gloves
Nitrile gloves are cheaper than latex gloves
Nitrile gloves are less resistant to punctures than latex gloves
Nitrile gloves are unsuitable for routine laboratory work
444) Which of these is PPE?
Fire extinguisher
First aid kit
Hand-washing station
Lab coat
445) Which of these gloves would offer the most protection in a laboratory?
1 mil nitrile gloves
3 mil latex gloves
5 mil latex gloves
5 mil nitrile gloves
446) What are the three basic protective measures against radiation?
Time, distance, and clothing
Time, distance, and exposure
Time, distance, and shielding
Time, distance, and speed
447) A colleague accidentally spills liquid nitrogen on their hands, resulting in minor frostbite to the fingers. What action should you take?
Immerse his hands in very hot water
Rub his hands vigorously using your own hands
Spray his hands with tepid water
Tell him to shake his hands vigorously
448) What should be your first action in the event of an acid burn?
Cover the burn with gauze moistened in sterile saline
Flush the burn with water
Go to the emergency department
Neutralise the acid with sodium bicarbonate
449) What is the minimum amount of time the eyes should be flushed with water in the event of a chemical splash?
1 minute
5 minutes
10 minutes
15 minutes
450) A lab technologist has spilled concentrated hydrochloric acid onto his clothing and skin, affecting a large portion of his body. After he has removed his clothing, what is the next thing he should do?
Apply a burn ointment to his skin
Pour baking soda onto his skin
Seek emergency medical assistance
Use the emergency safety shower
451) What should be your first step in the event of a needlestick injury?
Fill out an accident report form
Go to a doctor or hospital emergency department
Report the incident to your supervisor
Wash the wound with soap and water
452) What should be your first action if a patient's body fluid splashes into your eyes?
Call 111
Determine whether the patient has an infectious disease
Flush your eyes with water
Make an entry in the accident logbook
453) On what assumption are universal precautions based?
All chemicals may be carcinogenic
All specimens are potentially infectious
Handwashing is the best way to prevent the spread of infection
Not all equipment is sterile
454) Which type of PPE is required when dealing with a patient on airborne precautions?
Face shield
Goggles
N95 respirator
Surgical mask
455) Which biosafety level does a nonpathogenic strain of E. coli require?
BSL-1
BSL-2
BSL-3
BSL-4
456) Which type of hazard do universal precautions apply to?
Bloodborne pathogens
Dangerous chemicals
Environmental hazards
Radiation
457) Which link in the chain of infection does wearing gloves primarily interrupt?
Infectious agent
Mode of transmission
Portal of exit
Reservoir
458) What is the single most important method of preventing infection?
Proper handwashing
Sneezing into your arm
Thorough cleaning of equipment
Wearing a lab coat
459) Which two pictograms are needed on bottles of organic peroxides?
Flame and exploding bomb
Flame and flame over a circle
Health hazard and exclamation mark
Health hazard and gas cylinder
460) Which of these is NOT found on a safety data sheet?
Fire potential
Health hazards
Safe handling measures
Noise level
461) What is the correct way to lift a heavy object?
Bend at the knees, not at the waist
Hold the object away from your body
Let your back do the lifting
Use your arm muscles to do the lifting
462) Centrifugation separates components based on:
density
electrical charge
light absorption
molecular structure
463) A CSF specimen is centrifuged in a microhematocrit tube to give a supernatant. The supernatant is xanthochromic. What does this most likely indicate?
The patient had a recent brain bleed
The specimen has a low protein level
The spinal tap was traumatic
There are bacteria in the spinal fluid
464) Which centrifuge is balanced?
a
b
c
d
465) What is deionised water?
Water containing alkaline minerals
Water containing carbon dioxide
Water containing extra minerals
Water with ions removed
466) Which microscope is best for examining an unstained, wet preparation of cells before dehydration?
Brightfield
Electron
Fluorescence
Phase contrast
467) Which item of glassware is designed to deliver specific volumes?
Beaker
Erlenmeyer flask
Volumetric flask
Volumetric pipette
468) A pipette gives a reading of 4.9 mL, but the true value of the volume of liquid inside the pipette is 5.0 mL. What is the percent error?
2%
4%
6%
8%
469) Which pipette is the most accurate?
Bulb
Graduated
Pasteur
Volumetric
470) Why does volumetric glassware have the letters TC inscribed on it?
It can hold toxic chemicals
It contains a fixed volume
It delivers a fixed volume
It is Type C glassware
471) Which type of pipette is used to measure and dispense various volumes using calibration marks (graduations) along the entire length of the tube?
Disposable
Pasteur
Serological
Volumetric
472) Which cabinet has the highest personal, environmental, and specimen protection?
Class A biological safety cabinet
Class I biological safety cabinet
Class II biological safety cabinet
Class III biological safety cabinet
473) What type of filter in biological safety cabinets removes airborne particles such as bacteria and viruses?
Electrostatic
Fibreglass
HEPA
UV light
474) On fume hoods where sashes open vertically, you should work with the hood sash in the _____________ position.
highest possible
lowest possible
middle
most comfortable
475) What is the purpose of a chemical hygiene plan?
To ensure all chemicals are kept clean and uncontaminated
To inform workers of the chemicals used for cleaning
To maintain an inventory of the chemicals used in the laboratory
To protect employees from dangerous chemicals
476) Which equipment is used to measure large amounts of liquid to make reagents?
Centrifuge
Pipette
Test tube
Volumetric flask
477) The thermometer of a water bath reads 37.4°C. What is the resolution of the thermometer?
0.1°C
0.4°C
1°C
4°C
478) What is the term for a document that identifies hazards and the ways to minimise their impact?
Accident and incident recording form
First aid assessment form
Inspection report
Risk assessment
479) UV lamps in biological safety cabinets should be turned on only when:
a technician is working in the cabinet
ethanol is still wet on surfaces
no one can be exposed to the UV light
the sash is fully open
480) A specimen in a biohazard bag arrives leaking. What should you do?
Freeze the sample to seal the leak
Process the specimen as normal
Put on PPE and follow the spill/leak protocol
Wipe it clean and process it
481) What is the correct way to transport a gas cylinder in the laboratory?
By carrying it by hand with at least two people
By rolling it along the floor
On a flat-bed trolley
Strapped to a cart
482) What is the main purpose of reporting near-miss incidents?
To compare your department's safety record to that of other departments
To decide who to blame
To prevent the situation from recurring
To reduce employee evaluations
483) What is the name of the process of assessing the severity and likelihood of workplace hazards?
Health needs evaluation
Myers-Briggs type indicator
Risk assessment
Workplace danger evaluation
484) Which of these actions helps prevent the release of aerosols in the laboratory?
Filling centrifuge tubes to the brim
Forcibly expelling hazardous materials from pipettes
Opening aerosol containment devices immediately after removing them from the centrifuge
Routinely inspecting the centrifuge to ensure there is no leakage
485) What should be done in the event of a gas leak?
Alert others and evacuate the area
Light a match to find the source of the leak
Turn on all lights
Use elevators to exit the building
486) You receive a small cut from clean glassware. After the cut has stopped bleeding, what should you do?
Apply a tourniquet
Bandage it tightly
Clean the wound with soap and water
Soak the cut in disinfectant
487) What document should be completed after any workplace injury?
Incident report
Material Safety Data Sheet
Quality assurance audit
Standard Operating Procedure
488) Which of these would best promote a positive safety culture in a lab environment?
Allowing staff to ignore small risks
Encouraging open communication about hazards
Issuing penalties for every safety mistake
Reducing training time to save costs
489) Who is responsible for being aware of laboratory hazards?
The lab assistant
The lab manager
The lab director
Anyone who works in the laboratory
490) What should you do if a piece of laboratory equipment has a frayed electrical cord?
Continue using it carefully
Replace the cord
Report it to a supervisor
Tape over the damaged area
491) Which water purification method involves boiling water into steam, and then letting the steam cool?
Deionisation
Distillation
Filtration
Reverse osmosis
492) What is the function of a buffer?
To accentuate changes in pH
To make a solution more acidic
To make a solution more basic
To minimise changes in pH
493) Why must pipette tips be changed after each sample?
To prevent bubbles from forming in the pipette
To prevent cross-contamination between samples
To prevent wear and tear on the pipette
To reduce the time spent pipetting
494) What substance is used to calibrate refractometers?
Buffer solution
Distilled water
Healthy urine
Hydrochloric acid
495) Which of these chemical grades has the highest purity?
Commercial
Laboratory
Reagent
Technical
496) Which of these actions should a technologist take before working with unfamiliar equipment?
Ask another technologist to do it
Ask for proficiency testing
Look through the personnel files
Request training
497) Which of these actions would increase a laboratory's efficiency?
Finding ways to increase the time it takes to perform key tasks
Storing frequently used equipment in easy-to-reach places
Using an inconsistent labelling system
Using paper files instead of digital files
498) What is a delta check?
A comparison of a laboratory's results to those from an outside facility
A comparison of current test results to previous results
An inspection to ensure all patients are wearing wristbands
The verification of a patient's identity using at least three identifiers
499) A test tube solution has a concentration of 0.26 M, a path length of 2 cm, and an absorbance of 0.072 at 530 nm. To two significant digits, calculate the molar absorptivity.
0.04
0.14
1.61
1.81
500) How are acid buffers made?
By mixing a strong acid with a strong base
By mixing a strong acid with a weak base
By mixing a weak acid with its salt
By mixing a weak base with its salt
Access options
Get online access or buy a physical book.
Option 1: Online access
Unlock the answers to 500 questions for just $39.99
You'll get immediate access after payment.
Step 1
Enter the email address where you want us to send your order to.
Step 2
Select one of the payment buttons below.
Option 2: Physical book
Get the questions as a book
The questions and answers are available as a book at Amazon.com