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MLPAO MLA/T Certification Exam: Practice Test 2

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About the MLPAO MLA/T exam

The MLPAO MLA/T Certification Exam is a test that students must pass to work as laboratory assistants in Ontario, Canada. The exam is administered by the Medical Laboratory Professionals Association of Ontario (MLPAO).

About these practice questions

These practice questions will help prepare you for the MLPAO MLA/T exam.

This page contains 500 practice questions divided into the thirteen sections of the exam: 1. Standards of Practice, 2. Medical Terminology, 3. Basic Biology, Anatomy and Physiology, 4. Laboratory Mathematics and Quality Management, 5. Specimen Procurement, Processing and Data Collection, 6. Laboratory Safety, 7. Laboratory Equipment, 8. Histology and Cytology, 9. Clinical Microbiology, 10. Clinical Chemistry, 11. Clinical Hematology, 12. Transfusion Medicine, and 13. Electrocardiograms.

All questions have been carefully designed to mimic the questions on the real exam, to help you prepare and get a passing grade.

Check out all the practice tests in this series: Practice Test 1, Practice Test 2, and Practice Test 3.

Sections

  1. Standards of Practice
  2. Medical Terminology
  3. Basic Biology, Anatomy and Physiology
  4. Laboratory Mathematics and Quality Management
  5. Specimen Procurement, Processing and Data Collection
  6. Laboratory Safety
  7. Laboratory Equipment
  8. Histology and Cytology
  9. Clinical Microbiology
  10. Clinical Chemistry
  11. Clinical Hematology
  12. Transfusion Medicine
  13. Electrocardiograms

Section 1: Standards of Practice

1.1) Which principle of the Canada Health Act is not patient-focused but the means of achieving the other four principles?
  1. Accessibility
  2. Comprehensiveness
  3. Portability
  4. Public administration
  5. Universality
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1.2) Which of these is the responsibility of a laboratory assistant?
  1. Disciplining workers
  2. Giving results to patients
  3. Performing tests that require judgement
  4. Preparing chemical solutions
  5. Supervising other workers
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1.3) A lawyer asks a lab technician for copies of his client's test results. What should the lab technician do?
  1. Allow the lawyer to see the results on the computer
  2. Give the results to the lawyer immediately
  3. Have the lawyer sign a Medical Release Form and then release the results
  4. Have the lawyer sign a confidentiality form and then release the results
  5. Tell the lawyer to contact the physician who ordered the tests
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1.4) A phlebotomist draws blood for a pregnancy test. After the woman has left the room, a colleague approaches the phlebotomist and asks what test was ordered. He explains he wants to know because he is the woman's ex-boyfriend. The phlebotomist should:
  1. call the police
  2. inform his colleague he has no right to know which test was ordered
  3. lie and tell his colleague the test was a complete blood count
  4. reassure his colleague that he has nothing to worry about
  5. tell his colleague the test was a pregnancy test
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1.5) Another term for professional negligence is:
  1. defamation
  2. malfeasance
  3. malpractice
  4. misfeasance
  5. nonfeasance
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1.6) What is Canada's federal data privacy law for private-sector organizations?
  1. HIPPA
  2. PHIPA
  3. PIPA
  4. PIPEDA
  5. WHMIS
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1.7) Who enforces PIPEDA?
  1. Health Canada
  2. Information and Privacy Commissioner of Ontario
  3. Ministry of Health
  4. Privacy Commissioner of Canada
  5. Public Health Ontario
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1.8) The Regulated Health Professions Act, 1991 is legislation that:
  1. establishes the conditions that health insurance plans must meet to receive cash contributions
  2. governs Ontario's regulated health professions' Colleges
  3. governs the personal information handling practices of federal government institutions
  4. outlines the behaviour expected of health professionals
  5. sets the primary objective of Canadian health care policy
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1.9) Which Ontario act sets the rights and duties of workers and establishes the procedures for dealing with workplace hazards?
  1. Excellent Care for All Act
  2. Health Care Consent Act
  3. Laboratory and Specimen Collection Centre Licensing Act
  4. Occupational Health and Safety Act
  5. Regulated Health Professions Act
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1.10) In Ontario's Occupational Health and Safety Act, a 'competent person' is a person who:
  1. can make informed decisions about his or her health care
  2. follows the current CSMLS Standards of Practice guidelines
  3. has worked in their position for more than 10 years
  4. has worked in their position for more than 5 years
  5. is qualified because of knowledge, training, and experience
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1.11) Which of these is a 'controlled act' within the Regulated Health Professions Act (RHPA)?
  1. Drawing blood from a patient
  2. Praying for a patient
  3. Shaking a patient's hand
  4. Sneezing into your arm
  5. Weighing a patient
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1.12) The Health Care Consent Act is about patient consent to:
  1. confidentiality
  2. disclose health information
  3. privacy
  4. publication of their data in medical journals
  5. treatment
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1.13) Which government organization legislates the Laboratory and Specimen Collection Centre Licensing Act?
  1. Canadian Healthcare Association
  2. College of Medical Laboratory Technologists of Ontario
  3. Ministry of Health
  4. Ontario Health
  5. Public Health Agency of Canada
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1.14) According to Regulation 682 of the Laboratory and Specimen Collection Centre Licensing Act, a medical lab technician is a person:
  1. responsible for administrating technical and scientific lab operations
  2. responsible for supervising other workers in the laboratory
  3. who may perform specialized scientific tests without supervision
  4. who performs tests that require independent judgement
  5. who performs tests that require limited skills and responsibility
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1.15) What does PHIPA do?
  1. Control the use of confidential information at the federal level
  2. Control the use of confidential information at the provincial level
  3. Manage informed consent
  4. Protect employees
  5. Protect minorities
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1.16) What is the purpose of the Canada Health Act?
  1. To ensure all Canadian residents have access to necessary health care
  2. To ensure all health workers in Canada work in a safe environment
  3. To eradicate dangerous diseases from Canada
  4. To protect confidential patient information
  5. To provide health care services for people who can pay for the services
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1.17) The accessibility principle of the Canada Health Act states:
  1. Canadians must have reasonable access to healthcare facilities
  2. a Canadian who moves to a different province is still entitled to coverage
  3. all printed health materials must be made accessible in Braille
  4. anyone in Canada can register for free healthcare, regardless of immigration status
  5. healthcare facilities must be accessible to people with disabilities
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1.18) Which PIPEDA principle allows anyone to ask organizations for a copy of their personal information?
  1. Accountability
  2. Consent
  3. Individual Access
  4. Openness
  5. Safeguards
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1.19) Which act is Canada’s federal legislation for publicly funded health care insurance?
  1. Canada Health Act
  2. Established Programs Financing Act
  3. Hospital Insurance and Diagnostic Services Act
  4. Insurance Companies Act
  5. Medical Care Act
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1.20) Under the Canadian Constitution, which level of government is responsible for the direct health care services of First Nations living on reserves, Inuit and Innu populations, veterans, and inmates of federal prisons?
  1. Local
  2. District
  3. Municipal
  4. Provincial and territorial
  5. Federal
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Section 2: Medical Terminology

2.1) What does hypertrophy mean?
  1. Excess energy
  2. Increase in cell numbers
  3. Increase in size
  4. Newborn
  5. Underdeveloped
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2.2) What is the term for the contraction phase of the heartbeat?
  1. Diastole
  2. Pacemaker
  3. Septum
  4. Systole
  5. Tachycardia
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2.3) What is phagocytosis?
  1. An abnormally high number of phagocytes in the blood
  2. An abnormally low number of phagocytes in the blood
  3. An autoimmune disease where phagocytes attack the body's own cells
  4. The process by which phagocytes engulf foreign material
  5. The process by which phagocytes move
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2.4) What is the common abbreviation for the Papanicolaou test?
  1. D-dimer test
  2. P-test
  3. PPN test
  4. Pap test
  5. Polymerase test
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2.5) Glycolysis is the breakdown of:
  1. glucose
  2. glycogen
  3. glucagon
  4. glycerol
  5. glycosylate
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2.6) What is the term for the production of blood cells and platelets?
  1. Apheresis
  2. Apheresynthesis
  3. Coagulation
  4. Hemopoiesis
  5. Hemosynthesis
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2.7) White blood cells are also known as:
  1. erythrocytes
  2. leukocytes
  3. lipocytes
  4. osteocytes
  5. thrombocytes
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2.8) A thrombus is a:
  1. instrument for measuring blood pressure
  2. moving foreign body in the bloodstream
  3. type of blood clot
  4. type of blood vessel
  5. type of needle for aspirating pleural fluid
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2.9) What does the prefix brady- mean?
  1. Different
  2. Hidden
  3. Same
  4. Slow
  5. Together
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2.10) Which does the prefix adeno- refer to?
  1. Glands
  2. Joints
  3. Kidney
  4. Liver
  5. Muscle
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2.11) What does the suffix -emia refer to?
  1. Blood condition
  2. Eating disorder
  3. Enlargement
  4. Paralysis
  5. Surgical removal
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2.12) Which suffix means "tumor" or "mass"?
  1. -algia
  2. -emia
  3. -oma
  4. -osis
  5. -tomy
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2.13) In clinical chemistry, what does PSA stand for?
  1. Partly-specific antibody
  2. Patient safety awareness
  3. Plasma-specific antibody
  4. Platelet-specific antigen
  5. Prostate-specific antigen
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2.14) QNS stands for:
  1. quality not satisfied
  2. quality not sufficient
  3. quantity not satisfied
  4. quantity not sufficient
  5. questionable node suspected
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2.15) ETS stands for ________ ________ system.
  1. electrical tube
  2. evacuated tube
  3. extra thrombosin
  4. extravascular testing
  5. extravascular tube
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2.16) In the context of healthcare, what does CBC stand for?
  1. Center for blood care
  2. Center for blood control
  3. Complete blood count
  4. Complete blood culture
  5. Controlled blood count
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2.17) Small red or purple spots caused by a minor hemorrhage are called:
  1. palachiae
  2. parachiae
  3. parasites
  4. pasachiae
  5. petechiae
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2.18) What is edema?
  1. A bruise on the head
  2. A build-up of wax in the ear
  3. A collection of blood outside blood vessels
  4. Swelling caused by fluid retention
  5. The final product of the blood coagulation step in hemostasis
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2.19) What is a fistula?
  1. A build-up of pus in body tissue
  2. A knot of old scar tissue
  3. A vein that has become distended and engorged
  4. An abnormal connection between two parts of the body
  5. An infection of the large intestine
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2.20) What is a hematoma?
  1. A cancer of the blood that originates in red blood cells
  2. A cut in the skin
  3. A localized collection of blood outside the blood vessels
  4. A part of a blood clot that breaks off and travels to a new part of the body
  5. A severed artery
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2.21) What is hematuria?
  1. A clotting disorder
  2. A skin rash
  3. Bacteria in the bloodstream
  4. Blood in the urine
  5. Kidney stones
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2.22) What is the medical term for a low level of glucose in the blood?
  1. Hyperglycemia
  2. Hyperinsulinemia
  3. Hyperlipidemia
  4. Hypodermia
  5. Hypoglycemia
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2.23) What term refers to the replacement of normal parenchymal tissue with connective tissue?
  1. Alopecia
  2. Erythema
  3. Fibrosis
  4. Mucositis
  5. Myelosuppression
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2.24) What is the medical term for a high lymphocyte count?
  1. Lymphedema
  2. Lymphocytopenia
  3. Lymphocytosis
  4. Lymphoid
  5. Lymphopoiesis
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2.25) Which disease is the abnormal growth of bone, usually due to chronic kidney disease?
  1. Myelopoiesis
  2. Osteoclasis
  3. Osteodystrophy
  4. Osteoplasty
  5. Osteosis
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2.26) What is the medical term for low levels of oxygen in the blood?
  1. Hematemesis
  2. Hemoptysis
  3. Hemorrhage
  4. Hypoxemia
  5. Paroxysmal
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2.27) Hyperkalemia means high levels of which chemical element?
  1. Calcium
  2. Mercury
  3. Phosphorus
  4. Potassium
  5. Sodium
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2.28) What is the medical term for no urine production?
  1. Anuria
  2. Diuresis
  3. Micturition
  4. Nocturia
  5. Voiding
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2.29) The excision of a tissue sample for examination under a microscope is called a(n):
  1. abscess
  2. arthroscopy
  3. biopsy
  4. curettage
  5. laceration
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2.30) What is diastole?
  1. A heart rate above 120 beats per second
  2. A heart rate below 60 beats per second
  3. An irregular heart rate
  4. When the heart muscle contracts
  5. When the heart muscle relaxes
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2.31) The normal range for a test is known as the:
  1. coefficient of variation
  2. reference range
  3. specificity
  4. standard deviation
  5. standard range
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2.32) What is the medical term for an abnormally low urine volume?
  1. Aciduria
  2. Azoturia
  3. Oliguria
  4. Polyuria
  5. Pyuria
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2.33) What is the term for an elevated level of white blood cells in urine?
  1. Hematuria
  2. Nephritis
  3. Pyelonephritis
  4. Pyuria
  5. Uremia
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2.34) What is erythropoiesis?
  1. A red blood cell transfusion
  2. Decreased tissue demand for oxygen
  3. Hypoxia of cells that produce erythropoietin
  4. The destruction of red blood cells
  5. The process of red blood cells production
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2.35) A hospitalized patient has this sign above their bed: What does the sign mean?
  1. Do not intubate
  2. Do not resuscitate
  3. No blood draws allowed
  4. No food or drink allowed
  5. The patient is not allowed out of bed
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Section 3: Basic Biology, Anatomy and Physiology

3.1) What is the basic structural and functional unit of the human body?
  1. Cell
  2. DNA
  3. Organ
  4. System
  5. Tissue
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3.2) What is the major cation found in extracellular fluid?
  1. Chloride
  2. Magnesium
  3. Potassium
  4. Sodium
  5. Zinc
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3.3) Which system is a complex network of glands and organs that uses hormones to control and coordinate metabolism, growth, development, and mood?
  1. Digestive
  2. Endocrine
  3. Lymphatic
  4. Nervous
  5. Reproductive
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3.4) Oxygen and nutrients pass from the blood to the tissues via the:
  1. arteries
  2. arterioles
  3. capillaries
  4. lymphatic vessels
  5. veins
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3.5) What is the sequence of hemostasis?
  1. Coagulation, platelet plug formation, vasoconstriction
  2. Coagulation, vasoconstriction, platelet plug formation
  3. Platelet plug formation, coagulation, vasoconstriction
  4. Vasoconstriction, coagulation, platelet plug formation
  5. Vasoconstriction, platelet plug formation, coagulation
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3.6) Night blindness is caused by a deficiency in which vitamin?
  1. Vitamin A
  2. Vitamin B12
  3. Vitamin B6
  4. Vitamin C
  5. Vitamin D
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3.7) Jaundice is caused by a buildup of ___________ in the blood.
  1. bacteria
  2. bilirubin
  3. hemoglobin
  4. potassium
  5. uric acid
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3.8) Which process involves coating pathogens with antibodies to increase their susceptibility to phagocytosis?
  1. Agglutination
  2. Antiperistalsis
  3. Chemotaxis
  4. Complement activation
  5. Opsonization
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3.9) Complement can be activated through three pathways: classical, lectin, and:
  1. alternative
  2. concurrent
  3. contemporary
  4. modern
  5. tertiary
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3.10) Which cells produce histamine in a type 1 hypersensitivity reaction?
  1. Lymphocytes
  2. Macrophages
  3. Mast cells
  4. Neutrophils
  5. T cells
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3.11) Which bone is the femur?
  1. a
  2. b
  3. c
  4. d
  5. e
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3.12) The larger of the two lower leg bones is called the:
  1. condoyle
  2. cranial
  3. fibula
  4. tibia
  5. ulna
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3.13) Which fibrous connective tissue connects muscle to bone?
  1. Cartilage
  2. Ligament
  3. Muscle fibre
  4. Nerve
  5. Tendon
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3.14) Which hormone is also known as epinephrine?
  1. Adrenaline
  2. Cortisol
  3. Oxytocin
  4. Testosterone
  5. Vasopressin
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3.15) Which gland secretes cortisol?
  1. Adrenal
  2. Pineal
  3. Pituitary
  4. Thymus
  5. Thyroid
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3.16) Which gland produces growth hormone?
  1. Adrenal
  2. Endocrine
  3. Pancreas
  4. Parathyroid
  5. Pituitary
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3.17) Which hormone is antagonistic to insulin?
  1. Calcitonin
  2. FSH
  3. Glucagon
  4. Thyroxine
  5. Vasopressin
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3.18) The thyroid gland is located next to the:
  1. brain
  2. kidneys
  3. larynx
  4. liver
  5. spleen
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3.19) Which gland produces thyroid-stimulating hormone?
  1. Adrenals
  2. Parathyroids
  3. Pituitary
  4. Testes
  5. Thyroid
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3.20) What is the functional unit of the kidney?
  1. Alveoli
  2. Glomerular tuft
  3. Glomerulus
  4. Loop of Henle
  5. Nephron
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3.21) In the kidneys, substances travel from the glomerulus into the Bowman capsule by which process?
  1. Active transport
  2. Diffusion
  3. Evaporation
  4. Filtration
  5. Osmosis
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3.22) What are the end products of protein digestion?
  1. Amino acids
  2. Enzymes
  3. Fatty acids
  4. Monosaccharides
  5. Triglycerides
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3.23) The end products of fat digestion are fatty acids and:
  1. alkaloids
  2. amino acids
  3. glucose
  4. glycerol
  5. sebum
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3.24) After leaving the stomach, food next enters the:
  1. esophagus
  2. large intestine
  3. liver
  4. rectum
  5. small intestine
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3.25) The small intestine consists of the duodenum, jejunum and:
  1. anal canal
  2. cecum
  3. colon
  4. ileum
  5. rectum
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3.26) Nerve cells are also called:
  1. axons
  2. dendrites
  3. glial cells
  4. neuroglial cells
  5. neurons
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3.27) Which part of a neuron carries impulses away from the neuron?
  1. Axon
  2. Cell body
  3. Dendrite
  4. Myelin sheath
  5. Schwann cell
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3.28) The central nervous system includes the:
  1. brain and spinal cord
  2. heart
  3. peripheral nerves
  4. somatic nerves
  5. spinal nerves
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3.29) The impulse in a neuron moves from the ____________ to the ____________.
  1. Schwann cell, myelin
  2. axon, dendrite
  3. cell body, myelin
  4. dendrite, axon
  5. myelin, Schwann cell
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3.30) What are the smallest blood vessels in the body?
  1. Arteries
  2. Capillaries
  3. Lymph nodes
  4. Lymph vessels
  5. Veins
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3.31) Which part of the heart sets the heart rate?
  1. AV node
  2. Left atrium
  3. Right atrium
  4. Sinoatrial node
  5. VA node
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3.32) On which part of the body is the basilic vein?
  1. Arm
  2. Foot
  3. Hand
  4. Leg
  5. Neck
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3.33) Which is the largest artery in the body?
  1. Aorta
  2. Carotid artery
  3. Iliac artery
  4. Pulmonary artery
  5. Superior vena cava
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3.34) Refer to the image below: Which line is pointing to the left atrium?
  1. a
  2. b
  3. c
  4. d
  5. e
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3.35) From the left atrium, blood next flows into the:
  1. aorta
  2. left lung
  3. left ventricle
  4. right atrium
  5. right ventricle
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3.36) Which of these is a vein in the arm?
  1. Femoral
  2. Jugular
  3. Median cubital
  4. Portal
  5. Radial
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3.37) Which skin structure helps prevent water loss and inhibits bacterial growth on the surface of the skin?
  1. Arrector pili
  2. Hair follicles
  3. Nerves
  4. Oil glands
  5. Sweat glands
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3.38) The dermis is:
  1. above the epidermis
  2. also known as subcutaneous tissue
  3. also known as the epithelial layer
  4. the basal layer of the skin
  5. the middle layer of the skin
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3.39) In which organelle does ATP production occur?
  1. Flagellum
  2. Golgi apparatus
  3. Mitochondrion
  4. Nucleus
  5. Rough endoplasmic reticulum
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3.40) Which cellular component is involved in packaging enzymes?
  1. Centrioles
  2. Endoplasmic reticulum
  3. Golgi apparatus
  4. Mitochondria
  5. Ribosomes
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3.41) What are the three major disaccharides?
  1. Glucose, galactose, and fructose
  2. Glucose, galactose, and lactose
  3. Glucose, lactose, and sucrose
  4. Lactose, fructose, and galactose
  5. Sucrose, lactose, and maltose
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3.42) What is the main detoxification organ?
  1. Adenoids
  2. Kidneys
  3. Liver
  4. Pancreas
  5. Spleen
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3.43) Which organ is the line pointing to?
  1. Bladder
  2. Gallbladder
  3. Liver
  4. Pancreas
  5. Stomach
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3.44) Which enzyme unwinds DNA?
  1. Helicase
  2. Hexonuclease
  3. Ligase
  4. Primase
  5. Topoisomerase
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3.45) DNA polymerase can synthesize:
  1. DNA in the 3' to 5' direction
  2. DNA in the 5' to 3' direction
  3. RNA in the 3' to 5' direction
  4. RNA in the 5' to 3'direction
  5. mRNA in the 3' to 5' direction
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3.46) How many nucleotides are in a codon?
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
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3.47) In DNA, which base pairs with cytosine?
  1. Cytosine
  2. Guanine
  3. Thymine
  4. Uracil
  5. Xanthine
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3.48) Antibody class is determined by the:
  1. constant region of the heavy chain
  2. constant region of the light chain
  3. disulphide bonds
  4. variable region of the heavy chain
  5. variable region of the light chain
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3.49) High levels of which lipoprotein are associated with a decreased risk of accelerated atherosclerosis?
  1. Chylomicrons
  2. HDL
  3. Insulin
  4. LDL
  5. VLDL
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3.50) The simplest form of carbohydrate is called a:
  1. free fatty acid
  2. glycerol molecule
  3. monosaccharide
  4. nucleotide
  5. protein
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3.51) Lactose is a disaccharide of which two monosaccharides?
  1. Fructose and galactose
  2. Glucose and fructose
  3. Glucose and galactose
  4. Glucose and glucose
  5. Glucose and xylose
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3.52) Which organ does hydrogen breath testing investigate?
  1. Bladder
  2. Intestine
  3. Liver
  4. Lungs
  5. Stomach
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3.53) Which blood cells defend the body against disease?
  1. Erythrocytes
  2. Hematocytes
  3. Leukocytes
  4. Osteocytes
  5. Thrombocytes
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3.54) Which component of blood carries oxygen?
  1. Antigens
  2. Granulocytes
  3. Hemoglobin
  4. Platelets
  5. White blood cells
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3.55) What is this blood cell?
  1. B cell
  2. Macrophage
  3. Neutrophil
  4. Platelet
  5. Red blood cell
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3.56) Hematopoiesis occurs mainly in the:
  1. blood
  2. bone marrow
  3. brain
  4. liver
  5. pancreas
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3.57) A reticulocyte is:
  1. a mature red blood cell
  2. an immature erythrocyte
  3. demonstrated using a Wright-Giemsa stain
  4. found only in the bone marrow
  5. part of the reticuloendothelial system
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3.58) Phagocytes:
  1. carry carbon dioxide around the body
  2. carry oxygen around the body
  3. form clots to stop bleeding
  4. ingest pathogens
  5. release antibodies against pathogens
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3.59) Which of these white blood cells is a phagocyte?
  1. B cell
  2. Basophil
  3. Eosinophil
  4. Neutrophil
  5. T cell
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3.60) What are the two main types of lymphocytes?
  1. B cells and T cells
  2. Cytotoxic cells and helper cells
  3. Granulocytes and agranulocytes
  4. Macrophages and thrombocytes
  5. Plasma cells and memory cells
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3.61) Which of these cells is a granulocyte?
  1. Dendritic cell
  2. Macrophage
  3. Natural killer T cell
  4. Natural killer cell
  5. Neutrophil
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3.62) The three types of granulocyte are neutrophils, eosinophils, and:
  1. basophils
  2. erythrocytes
  3. lymphocytes
  4. monocytes
  5. thrombocytes
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3.63) Which blood cell is typically the first to appear in the response to an infection?
  1. Basophil
  2. Eosinophil
  3. Eosinophil
  4. Erythrocyte
  5. Neutrophil
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3.64) Which test can diagnose HIV in the early stages of infection, before antibodies are detectable?
  1. Immunelectrophoresis
  2. Nucleic acid test (NAT)
  3. Ouchterlony double diffusion
  4. Radioimmunoassay (RIA)
  5. The Quellung test
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3.65) Which test detects antibodies attached to red blood cells?
  1. Antibody screen
  2. Direct antiglobulin test
  3. Elution
  4. Indirect antiglobulin test
  5. Radioallergosorbent (RAST) test
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Section 4: Laboratory Mathematics and Quality Management

4.1) A molar solution is:
  1. 1 mL of solute in 1 litre of solution
  2. 1 mL of solute in one mole of solution
  3. one gram of solute in 1 mL of solution
  4. one gram of solute in one mole of solution
  5. one mole of solute in 1 litre of solution
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4.2) What is the dilution factor 1/5 expressed as a dilution ratio?
  1. 1:4
  2. 1:5
  3. 1:6
  4. 5:1
  5. 5:4
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4.3) A lab technician is making a solution by combining 3 parts of a solute with 7 parts of water. How many millilitres of the solute and water are needed to make 100 mL of the solution?
  1. 7 mL of solute and 3 mL of water
  2. 10 mL of solute and 70 mL of water
  3. 30 mL of solute and 70 mL of water
  4. 70 mL of solute and 30 mL of water
  5. 300 mL of solute and 700 mL of water
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4.4) If a dilution ratio of 7:10 is used to make an acetic acid solution and the final volume of the solution is 950 mL, what is the volume of the solute?
  1. 58 mL
  2. 101 mL
  3. 391 mL
  4. 559 mL
  5. 665 mL
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4.5) A lab technician adds 0.2 mL of a stock solution to 3.8 mL of diluent. What is the dilution factor?
  1. 4
  2. 5
  3. 19
  4. 20
  5. 21
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4.6) 0.5 mol of solute is dissolved in 250 cm³ of solution. What is the concentration in mol/dm³?
  1. 0.002 mol/dm³
  2. 2 mol/dm³
  3. 12.5 mol/dm³
  4. 125 mol/dm³
  5. 500 mol/dm³
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4.7) What does the unit mol/kg represent?
  1. Milli-molarity
  2. Molality
  3. Molar concentration
  4. Molar mass
  5. Molarity
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4.8) How many grams of sodium chloride are needed to make 200 mL of a 5% m/v sodium chloride solution?
  1. 5 g
  2. 10 g
  3. 15 g
  4. 20 g
  5. 25 g
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4.9) How do you calculate the mass of a solute in a solution?
  1. Concentration / volume
  2. Concentration × volume
  3. Volume + concentration
  4. Volume − concentration
  5. Volume/concentration
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4.10) A lab technician requires 400 mL of a 3% solution but has only a 5% solution available. How would the lab technician prepare the 3% solution?
  1. Mix 240 mL of the 5% solution with 160 mL of diluent
  2. Mix 300 mL of the 5% solution with 100 mL of diluent
  3. Mix 360 mL of the 5% solution with 40 mL of diluent
  4. Mix 380 mL of the 5% solution with 20 mL of diluent
  5. Mix 388 mL of the 5% solution with 12 mL of diluent
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4.11) How many millilitres of an 80% acetic acid solution is needed to make 2 litres of a 10% acetic acid solution?
  1. 20
  2. 50
  3. 100
  4. 250
  5. 500
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4.12) How would you prepare 400 mL of a 1% v/v acetic acid solution?
  1. 1 mL of glacial acetic acid in 399 mL of water
  2. 2 mL of glacial acetic acid in 398 mL of water
  3. 3 mL of glacial acetic acid in 397 mL of water
  4. 4 mL of glacial acetic acid in 396 mL of water
  5. 40 mL of glacial acetic acid in 400 mL of water
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4.13) What does 5% w/v mean?
  1. 5 grams of solute dissolved in 100 mL of solution
  2. 5 grams of solute dissolved in 100 mL of solvent
  3. 5 grams of solution dissolved in 95 mL of solution
  4. 5 mL of solute dissolved in 100 mL of solution
  5. 5 mL of solute dissolved in 100 mL of solvent
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4.14) Calculate the %m/m of a solution that has 30 grams of solute and 90 grams of solvent.
  1. 8.3%
  2. 9.1%
  3. 11%
  4. 25%
  5. 31%
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4.15) Which of these expresses solution concentration for a solid solute dissolved in a liquid solvent?
  1. %(m/m)
  2. %(m/v)
  3. %(v/m)
  4. %(v/v)
  5. %(w/m)
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4.16) What is the formula for calculating a percent w/w solution?
  1. (solute mass / solution mass ) × 100
  2. (solute mass / solution volume) × 100
  3. (solution mass / solute mass) × 100
  4. (solution mass / solute volume) × 100
  5. (solution volume / solute volume) × 100
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4.17) A mg is a unit to describe:
  1. length
  2. mass
  3. speed
  4. time
  5. volume
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4.18) Which prefix means 10⁻⁶?
  1. Centi-
  2. Kilo-
  3. Micro-
  4. Milli-
  5. Nano-
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4.19) What does the prefix kilo- mean?
  1. 10
  2. 100
  3. 1,000
  4. 10,000
  5. 100,000
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4.20) Which prefix means 0.001?
  1. Micro-
  2. Centi-
  3. Pico-
  4. Milli-
  5. Nano-
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4.21) What is 40°F in Celsius and rounded to the nearest whole number?
  1. −19°C
  2. 4°C
  3. 21°C
  4. 37°C
  5. 52°C
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4.22) What is 100°C in Fahrenheit?
  1. 45°F
  2. 78°F
  3. 157°F
  4. 212°F
  5. 274°F
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4.23) Round 2.08691 to three decimal places.
  1. 2.08
  2. 2.086
  3. 2.087
  4. 2.089
  5. 2.09
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4.24) What is 89.9583 rounded to three significant figures?
  1. 89.8
  2. 89.9
  3. 89.958
  4. 90.0
  5. 91.0
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4.25) What is 1 dm³ in cm³?
  1. 0.001 cm³
  2. 0.01 cm³
  3. 0.1 cm³
  4. 10 cm³
  5. 1,000 cm³
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4.26) What is 0.05 mL in microlitres?
  1. 0.00005 μL
  2. 0.0005 μL
  3. 0.005 μL
  4. 5 μL
  5. 50 μL
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4.27) The chart below shows a patient's blood carbon dioxide levels over one week. What type of trend does the chart demonstrate?
  1. Exponential
  2. Linear
  3. Logarithmic
  4. Polynomial
  5. Power
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4.28) For data distributed normally, how many values are within two standard deviations from the mean?
  1. 50%
  2. 68%
  3. 83%
  4. 95%
  5. 99.7%
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4.29) The following data were calculated on a series of 100 determinations of a control.
Mean 5.8
Median 6.0
Mode 5.7
Range 1.5–20.2
Standard deviation 0.25
If confidence limits are set at ±2 standard deviations, what are the allowable limits for the control?
  1. 5.30–6.30
  2. 5.35–6.25
  3. 5.50–5.90
  4. 5.60–5.90
  5. 5.65–5.95
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4.30) A test has a mean of 72 mmol/L and a standard deviation of 3 mmol/L. If all control values must fall within 3 standard deviations of the mean, which of the following values is outside the acceptable range?
  1. 62 mmol/L
  2. 71 mmol/L
  3. 78 mmol/L
  4. 80 mmol/L
  5. 81 mmol/L
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4.31) A hospital wants to set a new reference range for fasting blood glucose. It measures the fasting blood glucose levels of 451 healthy patients. The mean is 4.7 mmol/L and the standard deviation is 0.4 mmol/L. Calculate a reference range that covers 95% of the patients.
  1. 0.4–4.7 mmol/L
  2. 3.7–5.7 mmol/L
  3. 3.9–5.5 mmol/L
  4. 4.3–5.1 mmol/L
  5. 4.3–5.7 mmol/L
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4.32) Which formula calculates the coefficient of variation?
  1. Mean / standard deviation
  2. Mean / variance
  3. Standard deviation / mean
  4. Standard deviation / variance
  5. Variance / mean
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4.33) Automated white blood cell counts were performed on 29 blood samples. The mean value was 8.0 cells/nL and the standard deviation was 1.2 cells/nL. What is the coefficient of variation?
  1. 15%
  2. 30%
  3. 45%
  4. 60%
  5. 75%
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4.34) What is the mean of the values 2, 2, 5, 7, and 14?
  1. 2
  2. 3
  3. 4
  4. 5
  5. 6
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4.35) The middle value in a sorted data set is called the:
  1. average
  2. coefficient of variation
  3. mean
  4. median
  5. mode
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4.36) If you add up all the values in a set and then divide the total by the number of values, you get the:
  1. coefficient of variation
  2. mean
  3. median
  4. mode
  5. standard deviation
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4.37) Tube A contains 24 mL of blood. Tube B contains 20 mL of blood. This means tube A contains ____% more blood than tube B.
  1. 4
  2. 16
  3. 20
  4. 25
  5. 32
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4.38) Which grade of chemical is the highest purity?
  1. Commercial
  2. Laboratory
  3. Purified
  4. Reagent
  5. Technical
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4.39) Which of these is a post-analytical error?
  1. A hemolyzed specimen
  2. A test performed on the wrong patient
  3. An empty collection tube
  4. Incorrect information on the test request
  5. Results reported in the wrong units
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4.40) Which ISO standard specifies requirements for quality and competence in medical laboratories?
  1. ISO 15106
  2. ISO 15189
  3. ISO 15503
  4. ISO 15513
  5. ISO 15520
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4.41) Which of these is a pre-analytical quality indicator?
  1. Critical value reporting time
  2. Number of improperly labelled samples
  3. Number of laboratory reporting errors
  4. Percentage of results delivered late
  5. Turnaround time
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4.42) Which of the following is an example of a quality assurance indicator?
  1. C-reactive protein test
  2. Emergency procedures
  3. Keep thorough records of exposure incidents
  4. Laboratory staff must work under a fume hood when dealing with toxic chemicals
  5. Laboratory turnaround time
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4.43) Precision indicates the:
  1. closeness of a result to the first standard deviation
  2. closeness of a result to the median value
  3. closeness of a result to the mode
  4. closeness of a result to the true value
  5. repeatability or reproducibility of a procedure
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4.44) A new method for diagnosing fibromyalgia is trialled on 1,000 patients. The method detects 125 true positives, 25 false positives, 800 true negatives and 50 false negatives. Calculate the diagnostic specificity of the test.
  1. 91.9%
  2. 97.0%
  3. 98.2%
  4. 98.9%
  5. 99.3%
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4.45) A test correctly identifies 50% of sick people as sick and 100% of healthy people as healthy. The test shows:
  1. bias
  2. correlation
  3. deviation
  4. sensitivity
  5. specificity
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Section 5: Specimen Procurement, Processing and Data Collection

5.1) When drawing blood from a frightened child, you should:
  1. bribe the child with candy
  2. explain the procedure to the child in simple terms
  3. say nothing to the child and just perform the procedure
  4. tell the child that the needle won't hurt
  5. tell the child to be brave
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5.2) When drawing blood from an obese patient, which location should be the first place you look for a vein?
  1. The antecubital fossa
  2. The back of the hand
  3. The back of the knee
  4. The feet
  5. The neck
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5.3) A patient has an arteriovenous (AV) fistula in his left arm and an IV in his right arm. From where should you draw a blood sample?
  1. Above the AV fistula
  2. Above the IV
  3. Below the AV fistula
  4. Below the IV
  5. From the IV
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5.4) For venipuncture, tourniquets should be:
  1. applied very tightly to the arm
  2. left on the arm for at least three minutes
  3. removed after the needle is withdrawn
  4. tight enough to slow arterial flow
  5. tight enough to slow venous flow
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5.5) What is the most common antiseptic used to disinfect the site before venipuncture?
  1. 70% isopropyl alcohol
  2. Betadine
  3. Bleach
  4. EMLA
  5. Iodine
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5.6) When a blood pressure cuff is used for a venipuncture instead of a tourniquet, the pressure must be:
  1. between the patient's diastolic and systolic blood pressure
  2. equal to the patient's systolic blood pressure
  3. just above the patient's diastolic blood pressure
  4. just above the patient's systolic blood pressure
  5. just below the patient's diastolic blood pressure
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5.7) What can happen if a phlebotomist releases the tourniquet after removing the needle from the arm?
  1. Angina
  2. Bleeding
  3. Cyanosis
  4. Edema
  5. Paresthesia
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5.8) What can happen if an angle of 35 degrees is used when performing venipuncture?
  1. Nothing will happen as this is the correct angle
  2. The needle may go completely through the vein
  3. The needle may miss the vein completely
  4. The needle will enter above the vein
  5. The sample may be rejected due to hemolysis
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5.9) What should you do if a patient faints during a venipuncture?
  1. Continue the procedure until all blood is collected
  2. Leave the needle in the vein and call the physician
  3. Remove the needle and attend to the patient
  4. Start artificial respiration immediately
  5. Yell loudly at the patient to keep him conscious
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5.10) In venipuncture, which of these actions may cause a hematoma?
  1. Applying pressure to the puncture site after removing the needle
  2. Asking the patient to form a fist so the veins are more prominent
  3. Drawing blood from an arm that has an IV tube
  4. Inserting the needle through the vein and puncturing the opposite wall
  5. Removing the tourniquet before the needle is removed
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5.11) Which of these is a sign of a needle puncturing an artery during venipuncture?
  1. Blood spurting into the tube
  2. Breathing difficulties
  3. Dark blue blood
  4. Dark yellow blood
  5. Intense pain
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5.12) A phlebotomist is performing a venipuncture. The needle is in place but no blood is entering the tube. What is the first thing the phlebotomist should do?
  1. Change to pediatric tubes
  2. Discontinue the draw and cancel the requisition
  3. Push the needle in more deeply
  4. Switch to a winged blood collection set
  5. Try adjusting the needle slightly
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5.13) A physician requests a vitamin A test and a complete blood count. In which vacutainer tubes should the phlebotomist collect the blood samples?
  1. Gold and lavender
  2. Gold and light blue
  3. Grey and gold
  4. Grey and lavender
  5. Grey and light blue
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5.14) What are cross-match tubes used for?
  1. Blood alcohol testing
  2. Blood compatibility testing
  3. Coagulation studies
  4. Glucose tests
  5. Trace element studies
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5.15) Green top vacutainers contain which anticoagulant?
  1. Citrate
  2. EDTA
  3. Fluoride
  4. Heparin
  5. Oxalate
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5.16) Which vacutainer tube is used for hemoglobin testing?
  1. Blue
  2. Green
  3. Grey
  4. Lavender
  5. Red
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5.17) Which anticoagulant is in grey top vacutainers?
  1. EDTA
  2. Heparin
  3. Potassium oxalate
  4. SPS
  5. Sodium polyanethole sulfonate
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5.18) The results of a comprehensive metabolic panel show decreased calcium levels and elevated potassium levels. All other results are normal. What is the most likely cause of the results?
  1. The patient is dehydrated
  2. The patient is taking excessive vitamin D supplements
  3. The sample was stored at room temperature
  4. The sample was taken from an arm with an intravenous drip
  5. The tube was contaminated with EDTA
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5.19) What is the glycolytic inhibitor in grey top tubes?
  1. EDTA
  2. Heparin
  3. Silica
  4. Sodium citrate
  5. Sodium fluoride
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5.20) Which vacutainer tube is used for trace metal analysis?
  1. Grey
  2. Light blue
  3. Orange
  4. Red
  5. Royal blue
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5.21) Which colour vacutainer tube is known as the "SPS tube"?
  1. Gold
  2. Green
  3. Lavender
  4. Pink
  5. Yellow
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5.22) Some royal blue top tubes contain _____________ as an anticoagulant.
  1. EDTA
  2. lithium heparin
  3. potassium oxalate
  4. sodium citrate
  5. sodium heparin
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5.23) Which vacutainer tube contains sodium citrate?
  1. Green
  2. Grey
  3. Light blue
  4. Pink
  5. Red (glass)
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5.24) Which vacutainer tube contains no additives?
  1. Grey
  2. Pink
  3. Red (glass)
  4. Red (plastic)
  5. SST
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5.25) Sodium fluoride preserves:
  1. cellulite
  2. cellulose
  3. glucose
  4. glycerin
  5. protein
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5.26) What vacutainer tube is used for coagulation studies?
  1. Black
  2. Green
  3. Grey
  4. Light blue
  5. White
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5.27) What vacutainer tube is used for reticulocyte counts?
  1. Green
  2. Grey
  3. Lavender
  4. Light blue
  5. Red
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5.28) A phlebotomist needs to collect blood in a grey top tube, a gold top tube, and a lavender top tube. What is the correct order to fill the tubes?
  1. Gold, grey, lavender
  2. Gold, lavender, grey
  3. Grey, gold, lavender
  4. Grey, lavender, gold
  5. Lavender, gold, grey
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5.29) Black top vacutainer tubes contain which anticoagulant?
  1. EDTA
  2. Lithium heparin
  3. Sodium citrate
  4. Sodium fluoride
  5. Sodium heparin
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5.30) What additive prevents glycolysis in blood samples?
  1. EDTA
  2. Heparin
  3. Potassium oxalate
  4. Sodium citrate
  5. Sodium fluoride
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5.31) A phlebotomist is performing venipuncture on a difficult vein using a butterfly. The phlebotomist has to collect an SST and a light blue top tube. How should the phlebotomist proceed?
  1. Collect the SST first then the light blue tube
  2. Collect the light blue tube first then the SST
  3. Draw a clear tube first, then collect the SST, then collect the light blue tube
  4. Draw a clear tube first, then collect the light blue tube, then collect the SST
  5. Draw half of the SST first, then collect the light blue tube, then finish the SST
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5.32) What is the main purpose of tiger top tubes?
  1. Preventing clots
  2. Preventing glycolysis
  3. Separating plasma from blood cells
  4. Separating serum from blood cells
  5. Speeding up the formation of blood clots
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5.33) Silica in vacutainer tubes:
  1. accelerates clotting
  2. destroys microorganisms
  3. inhibits thrombin
  4. preserves pH
  5. prevents glycolysis
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5.34) Yellow top vacutainer tubes contain which anticoagulant?
  1. Acid citrate dextrose
  2. Potassium sorbate
  3. Sodium benzoate
  4. Sodium nitrite
  5. Sulphur dioxide
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5.35) EDTA prevents blood clots by binding:
  1. calcium
  2. chloride
  3. plasma
  4. platelets
  5. red blood cells
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5.36) What causes evacuated tubes to fill with blood automatically?
  1. Arterial blood pressure
  2. Gravity
  3. Pressure from the tourniquet
  4. Thermal insulation
  5. Vacuum in the tube
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5.37) Which tool is needed to collect blood by syringe?
  1. Microhematocrit tube
  2. Multisample needle
  3. Transfer device
  4. Tube holder
  5. Winged infusion set
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5.38) Which of these needles has the largest diameter?
  1. 18-gauge
  2. 19-gauge
  3. 20-gauge
  4. 21-gauge
  5. 22-gauge
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5.39) The gauge size of a needle indicates the diameter of the needle's:
  1. bevel
  2. flange
  3. hub
  4. lumen
  5. shaft
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5.40) Which part of a needle enters the skin first?
  1. Bevel
  2. Flange
  3. Hub
  4. Lumen
  5. Shaft
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5.41) Multisample needles are typically available in which gauges?
  1. 18–20
  2. 20–22
  3. 22–24
  4. 24–26
  5. 26–28
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5.42) When should you label a blood collection tube?
  1. As soon as you receive the test order
  2. Just before the patient arrives
  3. Before you collect the specimen
  4. After you collect the specimen
  5. After the patient has left
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5.43) If a patient is having blood drawn for a prothrombin time test, you might expect:
  1. the patient to be fasting
  2. the patient to bleed for a long time
  3. the serum to be very lipemic
  4. to have difficulty filling the tube
  5. to have to mix the tube more
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5.44) Why are gauze pads better than cotton balls for holding pressure on a puncture site?
  1. Cotton balls can stick to the puncture site
  2. Cotton balls contain latex which is dangerous for people with latex allergies
  3. Cotton balls have a higher risk of causing a compression nerve injury
  4. Gauze pads are more sterile
  5. Gauze pads are significantly cheaper
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5.45) Immediately after blood collection, how many times must tubes be inverted to ensure proper mixing of blood and additives?
  1. 1 or 2
  2. 8 to 10
  3. 10 to 15
  4. 15 to 20
  5. 20 to 30
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5.46) Blood specimens for which test are placed in circles on special filter paper?
  1. Bilirubin
  2. Complete blood counts
  3. Malaria
  4. Phenylketonuria
  5. The isolation of fungi and mycobacteria
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5.47) When whole blood is centrifuged in a grey top tube, what does it separate into?
  1. Plasma, buffy coat and red blood cells
  2. Plasma, clot, and red blood cells
  3. Red blood cells, white blood cells, and platelets
  4. Serum, buffy coat and red blood cells
  5. Serum, clot, and red blood cells
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5.48) Which medication might cause a patient to bleed excessively from a venipuncture site?
  1. A herbal medicine
  2. An anticoagulant
  3. Birth control pills
  4. Insulin
  5. Medicine for high cholesterol
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5.49) What is "the order of draw"?
  1. An order given by a superior to draw blood from a patient
  2. The correct sequence of steps for taking blood by venipuncture
  3. The order blood tubes are filled
  4. The order blood vials are stored
  5. The order you select patients to draw blood from
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5.50) What is the best time to collect a sputum specimen?
  1. An hour before the patient has dinner
  2. In the late afternoon
  3. In the middle of the day
  4. Just after the patient wakes up
  5. Just before the patient goes to bed
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5.51) Which of these tests is the most likely to require chain-of-custody documentation?
  1. Blood culture
  2. Cross-match
  3. Drug screen
  4. TDM
  5. Triglycerides test
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5.52) PKU tests are performed routinely on:
  1. HIV patients
  2. cancer patients
  3. elderly patients
  4. new employees
  5. newborn infants
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5.53) Which of these is an acceptable preservative for the phosphorus 24-hour urine test?
  1. Chlorhexidine
  2. Hydrochloric acid
  3. Sodium bicarbonate
  4. Sodium carbonate
  5. Sodium hydroxide
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5.54) A patient is receiving a course of antibiotics. His last dose was at 2 pm. His next doses will be at 10 pm and 6 am. When is the best time to draw a blood culture?
  1. At 2 am
  2. At 6 am
  3. Immediately
  4. Just after his next dose
  5. Just before his next dose
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5.55) How many bottles are drawn for a blood culture set?
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
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5.56) Which of these actions is NOT performed when performing quality control of a glucometer?
  1. Apply a drop of blood to the test strip
  2. Check the test strips are in date
  3. Compare the result displayed by the glucometer with the expected value
  4. Insert a test strip into the glucometer
  5. Remove the test strip from the glucometer
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5.57) Which fingers are used for finger puncture?
  1. Little finger and ring finger
  2. Little finger and thumb
  3. Middle finger and ring finger
  4. Ring finger and index finger
  5. Ring finger and thumb
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5.58) Which part of an infant's foot is the safest area to perform a capillary puncture?
  1. a
  2. b
  3. c
  4. d
  5. e
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5.59) After a semen sample is collected, within how many hours must it be tested?
  1. 1 hour
  2. 6 hours
  3. 12 hours
  4. 24 hours
  5. 72 hours
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5.60) At what temperature are cryofibrinogen specimens transported?
  1. −20°C or lower
  2. 4–6°C
  3. 21°C
  4. 37°C
  5. 55°C
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5.61) Which of these specimens needs to be kept at 35–37°C?
  1. Blood culture
  2. Feces
  3. Sputum
  4. Throat swab
  5. Urine
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5.62) Hemolysis of a blood specimen would NOT affect the result of which of these tests?
  1. Haptoglobin
  2. Iron
  3. MCHC
  4. Red blood cell count
  5. Total hemoglobin
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5.63) Category B infectious substances must be shipped with a label that says:
  1. UN2814 – Infectious Substance, Category B, Affecting Humans
  2. UN3291 – Clinical Waste, Category B
  3. UN3373 – Biological Substance, Category B
  4. UN3549 – Communicable Disease, Category B, Affecting Humans
  5. UN3549 – Medical Waste, Category B, Affecting Humans
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5.64) Which of these specimens is sterile in healthy people?
  1. Blood
  2. Feces
  3. Gastrointestinal tract specimens
  4. Sputum
  5. Urine
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5.65) What colour is a positive result on the fecal occult blood test?
  1. Black
  2. Blue
  3. Green
  4. Red
  5. Yellow
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5.66) Serum and plasma both contain:
  1. clotting factors
  2. plasma proteins
  3. platelets
  4. red blood cells
  5. white blood cells
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5.67) The glycosylated hemoglobin test is used to diagnose which disease?
  1. Acidosis
  2. Diabetes
  3. Hemoglobin C disease
  4. Inflammation
  5. Renal disease
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Section 6: Laboratory Safety

6.1) "Insidious" hazards are hazards that:
  1. are easily overlooked
  2. are unpreventable
  3. happen quickly
  4. involve chemicals
  5. involve fumes
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6.2) What is the word for an inanimate object that can transmit infectious agents from one individual to another?
  1. Adenovirus
  2. Bacteria
  3. Fomite
  4. Germ
  5. Parasite
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6.3) A toxic chemical that affects the body by being absorbed into the blood is called a blood:
  1. agent
  2. antagonist
  3. antibody
  4. attacker
  5. panel
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6.4) How should you pick up an item of hot glassware?
  1. By pouring cold water on it to cool it down
  2. Using latex gloves
  3. With a rag or paper towels
  4. With tongs
  5. With your hands
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6.5) Which of these actions helps prevent the release of aerosols in the laboratory?
  1. Filling centrifuge tubes to the brim
  2. Forcibly expelling hazardous materials from pipettes
  3. Opening aerosol containment devices immediately after removing them from the centrifuge
  4. Routinely inspecting the centrifuge to ensure there is no leakage
  5. Using tubes with cracks in them
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6.6) Used needles should be discarded in:
  1. a biohazard autoclave bag
  2. a biohazard sharps container
  3. a glass disposal bucket
  4. a glass recycling bin
  5. the regular garbage
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6.7) Which of these is an example of passive fire protection?
  1. Auto-closing ventilation in fume cupboards
  2. Fire alarms
  3. Fire extinguishers
  4. Fire-resistant walls
  5. Sprinklers
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6.8) What is an upper flammability limit?
  1. The amount of fuel that will burn before the fuel burns itself out
  2. The highest concentration of a chemical that can catch fire
  3. The highest temperature at which a chemical can be stored safely
  4. The highest temperature at which a chemical fire will ignite
  5. The lowest temperature at which a chemical will ignite
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6.9) Refer to the image below: Another name for this type of fire extinguisher is a _________ fire extinguisher.
  1. ABC
  2. AFFF foam
  3. carbon dioxide
  4. class F
  5. wet powder
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6.10) At low concentrations, what does hydrogen sulfide smell like?
  1. Burnt sugar
  2. Cherries
  3. Rotten eggs
  4. Rotting meat
  5. Strong cheese
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6.11) What is the term for chemicals that cause birth defects in embryos and fetuses?
  1. Carcinogens
  2. Corrosives
  3. Immunogens
  4. Phenyls
  5. Teratogens
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6.12) A lab assistant accidentally drinks a beaker of Zenker's fluid. He says he feels a burning sensation in his mouth and throat. The lab manager phones 911. While waiting for the ambulance to arrive, which of these other first-aid measures should be taken?
  1. Ask the lab assistant to remove his contact lenses
  2. Give the lab assistant water to drink
  3. Place the lab assistant in the recovery position
  4. Take the lab assistant outside for fresh air
  5. Tell the lab assistant to rinse his eyes with water for several minutes
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6.13) Mixing bleach with acid produces water, salt and:
  1. arsenic trioxide
  2. chlorine gas
  3. hydrogen cyanide
  4. phosgene
  5. sodium cyanide
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6.14) What is the correct order of removal of PPE?
  1. Gloves, gown, goggles, mask
  2. Gown, goggles, gloves, mask
  3. Gown, mask, gloves, goggles
  4. Mask, gloves, goggles, gown
  5. Mask, gown, gloves, goggles
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6.15) In which of these situations should safety goggles be worn?
  1. During blood collections
  2. During reagent and specimen preparation
  3. When talking to members of the public
  4. While inspecting reagent supplies
  5. While transporting sealed waste containers
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6.16) Which of these helps protect against non-ionizing radiation?
  1. Biosafety cabinet
  2. Fire extinguisher
  3. Fume hood
  4. Goggles
  5. Laboratory-safe refrigerator
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6.17) The three basic protective measures against radiation are time, distance, and:
  1. exposure
  2. lead
  3. radiation suit
  4. shielding
  5. speed
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6.18) You find a colleague unconscious and not breathing. There is no one around to help. What is the first thing you should do?
  1. Call 911
  2. Find a defibrillator
  3. Give chest compressions
  4. Give mouth-to-mouth breaths
  5. Move your colleague into the recovery position
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6.19) What should you do if someone is having a seizure?
  1. Give chest compressions
  2. Give mouth-to-mouth breaths
  3. Hold them down
  4. Put something soft under their head
  5. Put something wooden in their mouth
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6.20) People at risk of a severe allergic reaction often carry devices that inject which medication?
  1. Acetaminophen
  2. Adrenaline
  3. Fluticasone
  4. Insulin
  5. Morphine
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6.21) When should you NEVER administer care to a casualty?
  1. When the person has stopped breathing
  2. When the person is an organ donor
  3. When the person is having a seizure
  4. When the person is unconscious
  5. When the person refuses care
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6.22) The recovery position is used for patients who are:
  1. breathing abnormally
  2. conscious and breathing normally
  3. unconscious and not breathing
  4. unconscious but breathing normally
  5. undergoing cardiac arrest
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6.23) If you suspect someone has suffered a stroke, what is the first thing you should do?
  1. Apply pressure to the affected area
  2. Ask the person to lie down
  3. Contact emergency services
  4. Loosen items of tight clothing
  5. Perform CPR
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6.24) You find a person lying on the floor. You suspect they have received an electric shock. What is the first thing you must do before treating them?
  1. Check if the person is breathing
  2. Check if the person is still in contact with the electrical current
  3. Check the person's pulse
  4. Make a note in the incident logbook
  5. Wrap the person in a thermal blanket
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6.25) In CPR, how many chest compressions should be given per second?
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
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6.26) Which of the following is the correct sequence of events in the chain of survival?
  1. Call 911, defibrillation, start CPR
  2. Call 911, start CPR, defibrillation
  3. Defibrillation, call 911, start CPR
  4. Defibrillation, start CPR, call 911
  5. Start CPR, call 911, defibrillation
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6.27) A lab technologist has spilled concentrated hydrochloric acid onto his clothing and skin, affecting a large portion of his body. After he has removed his clothing, the next thing he should do is:
  1. apply a burn ointment to his skin
  2. apply bandages
  3. pour baking soda onto his skin
  4. seek emergency medical assistance
  5. use the emergency safety shower
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6.28) A colleague has cut his lower arm and is bleeding. Which of the following is NOT an appropriate response?
  1. Apply firm pressure to the wound using sterile gauze
  2. Clean and dress the wound once the bleeding has stopped
  3. Place a tourniquet over the elbow joint
  4. Tell your colleague to keep his arm raised
  5. Wear gloves
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6.29) The Heimlich maneuver is used for:
  1. choking
  2. heart attack
  3. heat stroke
  4. poisoning
  5. syncope
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6.30) What is the first action to take if someone goes into cardiac arrest?
  1. Assess the airway
  2. Call 911
  3. Check the pulse
  4. Perform chest compressions
  5. Put the person into the supine position
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6.31) What is the single most important method of preventing infection?
  1. Proper handwashing
  2. Sneezing into your arm
  3. Thorough cleaning of equipment
  4. Wearing a lab coat
  5. Wearing protective goggles
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6.32) Universal precautions apply to which type of hazard?
  1. Bloodborne pathogens
  2. Dangerous chemicals
  3. Environmental hazards
  4. Fire
  5. Radiation
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6.33) What is post-exposure prophylaxis?
  1. A delayed allergic reaction to an allergen
  2. An auto-immune disease
  3. The inability to breathe after exposure to an allergen
  4. The protocol for cleaning a laboratory after the release of a dangerous pathogen
  5. Treatment after exposure to a pathogen to prevent infection from occurring
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6.34) Which of these is an OPIM (other potentially infectious material)?
  1. Feces
  2. Saliva
  3. Semen
  4. Urine
  5. Vomit
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6.35) The six links in the chain of infection are infectious agent, reservoir, portal of exit, mode of transmission, portal of entry, and:
  1. behaviour
  2. hospital-acquired infection
  3. reagent
  4. susceptible host
  5. vaccination
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6.36) Bottles of organic peroxides require which two pictograms?
  1. Corrosion and exclamation mark
  2. Flame and exploding bomb
  3. Flame and flame over a circle
  4. Health hazard and exclamation mark
  5. Health hazard and gas cylinder
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6.37) Gases under pressure have a pictogram of:
  1. a flame
  2. a flame over a circle
  3. a gas cylinder
  4. an exploding bomb
  5. an exploding test tube
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6.38) If the word 'Forbidden' is written in column 3 of a Hazardous Materials Table, it means the material should not be:
  1. discarded
  2. ingested
  3. picked up
  4. transported
  5. used
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6.39) Which cleaning product releases a toxic gas when mixed with urine?
  1. Chlorine bleach
  2. Hydrogen peroxide
  3. Isopropyl alcohol
  4. Povidone-iodine
  5. Sodium hydroxide
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6.40) Ethanol can NOT kill:
  1. E. coli
  2. Pseudomonas aeruginosa
  3. Salmonella typhosa
  4. fungi
  5. spores
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6.41) Which colour on the NFPA hazard diamond signals the degree of hazards to health?
  1. Blue
  2. Green
  3. Red
  4. White
  5. Yellow
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6.42) What is the NFPA colour code for flammable hazards?
  1. Blue
  2. Green
  3. Red
  4. White
  5. Yellow
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6.43) The cabinet with the highest personal, environmental, and specimen protection is the:
  1. class A biological safety cabinet
  2. class I biological safety cabinet
  3. class II biological safety cabinet
  4. class III biological safety cabinet
  5. fume hood
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6.44) What virus causes hepatitis C?
  1. HAV
  2. HBC
  3. HCC
  4. HCV
  5. HPC
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6.45) Class 7 materials in the Transportation of Dangerous Goods Act are:
  1. corrosive
  2. explosive
  3. flammable or combustible
  4. poisonous or infectious
  5. radioactive
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6.46) Specimens containing the smallpox virus must be transported as:
  1. Category A
  2. Category B
  3. Class 1
  4. Class 2
  5. exempt
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6.47) Which class of dangerous goods are flammable solids?
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
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6.48) What are the three GHS hazard classes?
  1. Biological, chemical, and radiation
  2. Electrical, fire, and chemical
  3. Health, physical, and environmental
  4. Physical, psychosocial, and psychological
  5. Slips, trips, and falls
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6.49) According to the GHS Hazard Classification, which of these is a type of physical hazard?
  1. Carcinogenicity
  2. Eye effects
  3. Flammable gases
  4. Skin corrosion
  5. Skin irritation
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6.50) According to the American National Standards Institute (ANSI), which sign indicates a potential hazard that could result in minor or moderate injury?
  1. Caution
  2. Danger
  3. Notice
  4. Risk
  5. Warning
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6.51) What worldwide system gives recommendations on how to label hazardous chemicals?
  1. GHS
  2. HCS
  3. HPR
  4. HazCom
  5. WHMIS
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6.52) Who or what does protective isolation protect?
  1. A patient
  2. Lab equipment
  3. Laboratory samples
  4. Medical personnel
  5. The general public
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Section 7: Laboratory Equipment

7.1) A lab assistant has just finished washing an item of glassware with water when the supervisor comes over and says she needs the glassware right away. To dry the glassware quickly, the lab assistant should rinse it with:
  1. acetic acid
  2. acetone
  3. hydrochloric acid
  4. hydrogen peroxide
  5. sodium nitrate
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7.2) What is the best way to clean glassware containing water-insoluble solutions?
  1. Rinse 2-3 times with ethanol then rinse 3-4 times with deionized water
  2. Rinse 3-4 times with deionized water
  3. Rinse with copious amounts of tap water then rinse 3-4 times with deionized water
  4. Wash with hot soapy water
  5. Wash with hot soapy water, rinse thoroughly with tap water, and then rinse 3-4 times with deionized water
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7.3) Which of the following plastics is autoclavable?
  1. High density polyethylene
  2. Low density polyethylene
  3. Polypropylene
  4. Polystyrene
  5. Polyurethane
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7.4) Which item of lab equipment sterilizes using pressurized steam?
  1. Alcohol
  2. Autoclave
  3. Dry heat sterilizer
  4. Fume hood
  5. Oven
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7.5) Autoclave tape changes colour when:
  1. organisms have been killed
  2. sterilization has taken place
  3. the autoclave is nearing the end of its warranty
  4. the autoclave reaches a specific temperature
  5. there are spores remaining
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7.6) Autoclaving uses what method of sterilization?
  1. Chemical
  2. Dry heat
  3. Moist heat
  4. Physical
  5. Radiation
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7.7) Which type of glass is autoclavable?
  1. Annealed
  2. Borosilicate
  3. Float
  4. Lead
  5. Soda-lime
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7.8) Which of these can NOT be autoclaved?
  1. Gloves
  2. Microbial growth media
  3. Organic solvents
  4. Stainless steel
  5. Tissue culture flasks
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7.9) A lab assistant is wrapping an instrument for autoclaving but discovers the wrapper has a hole. What should the lab assistant do?
  1. Get a new wrapper
  2. Ignore the hole and continue wrapping the instrument
  3. Repair the hole with adhesive tape
  4. Throw both the instrument and the wrapper away
  5. Throw the instrument away
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7.10) Which of these biomarkers can urine refractometers measure?
  1. The presence of nitrates in urine
  2. Urine colour
  3. Urine hemoglobin concentration
  4. Urine osmolality
  5. Urine specific gravity
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7.11) Which item of lab equipment uses high-speed rotation in a closed chamber?
  1. Autoclave
  2. Centrifuge
  3. Evaporator
  4. Hot plate
  5. Shaker
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7.12) Which of these is a container used for heating substances over a Bunsen burner?
  1. Beaker
  2. Crucible
  3. Erlenmeyer flask
  4. Funnel
  5. Graduated cylinder
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7.13) Which of the following solutions is used to calibrate pH meters?
  1. Buffer solution
  2. Deionized water
  3. Distilled water
  4. Sodium chloride solution
  5. Sodium hydroxide solution
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7.14) Type 1 glass is:
  1. aluminosilicate
  2. borosilicate
  3. fused silica
  4. lead
  5. soda-lime
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7.15) Automated blood cell counters work on which principle?
  1. Boyle
  2. Campbell
  3. Coulter
  4. Ferguson
  5. McKenzie
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7.16) Which water purification destroys microorganisms but does not filter out unwanted minerals and ions?
  1. Double distillation
  2. Ion exchange column
  3. Reverse osmosis
  4. Ultrafiltration
  5. Ultraviolet radiation
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7.17) Which part of a microscope adjusts the light intensity?
  1. Iris diaphragm
  2. Numerical aperture
  3. Objective lens
  4. Ocular lens
  5. Stage clip
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7.18) The ___________ is a measure of a microscope's ability to gather light and resolve fine specimen detail.
  1. contrast
  2. empty magnification
  3. lens power
  4. numerical aperture
  5. objective
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7.19) What part of a microscope focuses light onto the specimen?
  1. Aperture
  2. Condenser
  3. Diaphragm
  4. Objective lens
  5. Ocular lens
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7.20) Microscopes usually have which objective lenses?
  1. 10x, 40x, and 100x
  2. 10x, 50x, and 100x
  3. 20x, 40x, and 80x
  4. 20x, 50x, and 80x
  5. 20x, 50x, and 100x
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7.21) Which pipette is the most accurate?
  1. Bulb
  2. Graduated
  3. Pasteur
  4. Vacuum-assisted
  5. Volumetric
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7.22) Which of these glass types has the strongest resistance to heat?
  1. Borosilicate
  2. Crystal
  3. Lead
  4. Optical
  5. Soda-lime
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7.23) Volumetric glassware has the letters TC inscribed on it because it:
  1. can hold toxic chemicals
  2. contains a fixed volume
  3. delivers a fixed volume
  4. is Type C glassware
  5. is good at controlling temperatures
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7.24) What does "10 mL TD 25 SEC" on a pipette mean?
  1. The pipette can be filled with 10 mL in 25 seconds
  2. The pipette can deliver 10 mL in its primary tube and 25 mL in its secondary tube
  3. The pipette holds 10 mL and has a diameter of 25 mm
  4. The pipette will deliver 10 mL when left to drain for 25 seconds
  5. You should wait 25 seconds between filling and dispensing the pipette
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7.25) Which kind of centrifuge is used to spin capillary tubes?
  1. Angle head
  2. Cytospin
  3. Gas
  4. Microhematocrit
  5. Refrigerated
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7.26) Which kind of centrifuge has good cellular morphology preservation?
  1. Angle head
  2. Cytocentrifuge
  3. Microhematocrit
  4. Refrigerated
  5. Ultracentrifuge
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7.27) Cytocentrifuges are also known as:
  1. basic centrifuges
  2. cell centrifuges
  3. cytospins
  4. low-angle centrifuges
  5. ultracentrifuges
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7.28) Which type of centrifuge is optimized for spinning a rotor at very high speeds and is capable of generating acceleration as high as 1,000,000 g?
  1. Clinical centrifuge
  2. Cytocentrifuge
  3. High-speed centrifuge
  4. Microcentrifuge
  5. Ultracentrifuge
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7.29) What is the most common cause of excessive centrifuge vibration?
  1. Electrical interference
  2. Excessive temperature
  3. Unbalanced tubes
  4. Uneven bench surfaces
  5. Variable voltage
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7.30) The two electrodes of a pH meter are called the ___________________ electrodes.
  1. capacitive and potentiated
  2. dry and wet
  3. glass and reference
  4. negative and positive
  5. sealed and ground
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Section 8: Histology and Cytology

8.1) What is the term for formaldehyde dissolved in water?
  1. Acetone
  2. Cytospray
  3. Eosin
  4. Ethyl acetate
  5. Formalin
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8.2) In histology, which of these chemicals is used for fixation?
  1. Chloroform
  2. Formalin
  3. Increasing strengths of alcohol
  4. Paraplast
  5. Xylene
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8.3) Which of these actions will prevent the formation of acid formaldehyde hematin in formaldehyde solutions?
  1. Adding drops of cytochrome b5 reductase
  2. Keeping the solutions refrigerated
  3. Placing the solutions in 70% alcohol overnight
  4. Using buffered formalin
  5. Washing excess fixative overnight with water
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8.4) What is the correct order of steps to process tissue containing calcium?
  1. Decalcification, dehydration, fixation, clearing, infiltration
  2. Decalcification, dehydration, fixation, infiltration, clearing
  3. Dehydration, fixation, infiltration, decalcification, clearing
  4. Fixation, decalcification, dehydration, clearing, infiltration
  5. Fixation, dehydration, decalcification, infiltration, clearing
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8.5) What happens if tissue is left in xylene for more than three hours?
  1. The tissue becomes a milky colour
  2. The tissue becomes brittle
  3. The tissue becomes red
  4. The tissue swells in size
  5. The xylene becomes solid
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8.6) Paraffin wax should be used at ___________ its melting point.
  1. 5–10°C below
  2. 2–3°C below
  3. the same temperature as
  4. 2–3°C above
  5. 5–10°C above
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8.7) An advantage of paraffin wax embedding is:
  1. it preserves fats
  2. rapid diagnosis
  3. samples can be stored long-term
  4. sections can be cut ultra-thin
  5. tissue can be viewed without a microscope
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8.8) What is the melting point of Paraplast?
  1. 52–56°C
  2. 62–66°C
  3. 72–76°C
  4. 82–86°C
  5. 92–96°C
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8.9) Which microtome is used to prepare tissue sections for electron microscopy?
  1. Cryostat
  2. Hand microtome
  3. Rotary microtome
  4. Sledge microtome
  5. Ultramicrotome
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8.10) Which freezing technique is the coldest?
  1. Aerosol sprays
  2. Carbon dioxide gas
  3. Carbon dioxide ‘cardice'
  4. Isopentane cooled by liquid nitrogen
  5. Liquefied nitrogen
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8.11) A lab technician has cut a ribbon of sections from a tissue block. What should the lab technician do next?
  1. Add a few drops of a softening agent
  2. Float the sections on warm water
  3. Leave the sections at room temperature for 30 minutes
  4. Leave the sections in a freezer for an hour
  5. Submerge the sections in ethanol
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8.12) Diamond blades are needed to cut:
  1. bone
  2. brain tissue
  3. intestinal tissue
  4. muscle tissue
  5. skin tissue
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8.13) Wax sections are typically cut how thick during microtomy?
  1. 4–10 nm
  2. 40–100 nm
  3. 4–10 µm
  4. 4–10 mm
  5. 40–100 mm
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8.14) For electron microscopy, sections must be about _______ times thinner than sections for light microscopy.
  1. 2
  2. 20
  3. 200
  4. 2,000
  5. 20,000
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8.15) Before staining, paraffin sections must be:
  1. dewaxed
  2. left underwater for at least an hour
  3. refrigerated
  4. submerged in a chlorine solution
  5. warmed in an oven
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8.16) In the hematoxylin and eosin (H&E) staining method, the eosin stains:
  1. connective tissue blue
  2. cytoplasms pink
  3. microorganisms blue
  4. nuclei blue
  5. nuclei pink
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8.17) The most common mordant for H&E staining is:
  1. Alcian blue
  2. alum
  3. eosin
  4. hematoxylin
  5. sodium metabisulphite
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8.18) What cell structure turns blue in H&E staining?
  1. Cytoplasm
  2. Golgi apparatus
  3. Mitochondria
  4. Nuclei
  5. Reticulum
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8.19) __________ staining is the process of staining living cells that have been removed from the body.
  1. Differential
  2. Gram
  3. In vivo
  4. Positive
  5. Supravital
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8.20) When a smear is too red, neutrophil granules look:
  1. blue-red
  2. brilliant red
  3. green
  4. indistinct
  5. light blue
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8.21) Which cytological technique involves passing a urine sample through a biological filter containing pores of a specific diameter?
  1. Centrifugation
  2. Clean-catch
  3. Flow cytometry
  4. Millipore filtration
  5. Urine culture
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Section 9: Clinical Microbiology

9.1) What is the counterstain in Gram's technique?
  1. Acetone-alcohol
  2. Crystal violet
  3. Giemsa
  4. Iodine
  5. Safranin
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9.2) In acid-fast staining, what colour are acid-fast bacteria?
  1. Blue
  2. Green
  3. Red
  4. White
  5. Yellow
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9.3) When exposed to acid, acid-fast bacteria:
  1. decolourize at the same rate as other bacteria
  2. decolourize faster than other bacteria
  3. decolourize more slowly than other bacteria
  4. do not decolourize
  5. move faster
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9.4) Which of these is needed for a malaria test?
  1. Agar
  2. Capillary tube
  3. Clay sealant
  4. Glass slide
  5. Microhematocrit tube
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9.5) A sample for the respiratory syncytial virus (RSV) test is best collected using a:
  1. cough plate
  2. expectorated sputum
  3. nasal aspirate
  4. throat swab
  5. tracheal tube
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9.6) What colour does the indicator strip inside a GasPak chamber turn when oxygen is present?
  1. Black
  2. Blue
  3. Colourless
  4. Red
  5. Yellow
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9.7) Smears of cerebrospinal fluid (CSF) are prepared with:
  1. centrifuged CSF sediment
  2. filtered CSF sediment
  3. filtered CSF supernatant
  4. incubated CSF supernatant
  5. uncentrifuged CSF supernatant
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9.8) Which test detects antibodies to the Epstein-Barr virus?
  1. C-reactive protein test
  2. Erythrocyte sedimentation rate
  3. Influenza test
  4. Monospot test
  5. Rapid Group A Streptococcus test
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9.9) Skin scrapings are set up in microbiology to identify:
  1. Corynebacterium
  2. Escherichia coli
  3. Neisseria
  4. fungi
  5. small cell lung cancer
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9.10) What is the usual temperature for an incubator used for culture and sensitivity?
  1. 15–17°C
  2. 25–27°C
  3. 35–37°C
  4. 45–47°C
  5. 55–57°C
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9.11) Which fluorescent dye glows green under blue light?
  1. Alexa Fluor
  2. Chromomycin A3
  3. DAPI
  4. GFP
  5. Rhodamine
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9.12) Which of the following incubation conditions is used to grow Streptococcus pneumoniae?
  1. At 35–37°C, over 3 days in chocolate agar or MacConkey agar, with 20% H₂
  2. At 35–37°C, overnight in media containing blood and sugar, with 5−10% CO₂
  3. At 42°C, over 2 days in media containing electrolytes and peptone water, and under microaerophilic conditions
  4. At 42°C, over 3 days in Luria Bertani agar, and under anaerobic conditions
  5. At 42°C, over 3 days in mannitol salt agar, with 15–20% O₂
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9.13) Bacteria that are NOT acid-fast are termed:
  1. acid-slow
  2. acidophilic
  3. alkaline-fast
  4. alkaliphilic
  5. non-acid-fast
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9.14) What is the goal of streaking an agar plate?
  1. To accelerate the solidification of the agar medium
  2. To determine if the bacteria can ferment glucose, sucrose and/or lactose
  3. To determine the number of organisms in the sample
  4. To produce isolated colonies for further study
  5. To produce dense growth which covers the plate
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9.15) Which ingredient in Thayer-Martin agar suppresses the growth of fungi?
  1. Nystatin
  2. Polymyxin
  3. Colistin
  4. Vancomycin
  5. Agar
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9.16) What is reducing media used for in microbiology?
  1. Cultivating organisms that only grow in blood
  2. Growing anaerobic bacteria
  3. Isolating fungi and yeasts
  4. Reducing the pH of broth or agar
  5. Testing pathogens for antibiotic susceptibility
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9.17) Which enzyme is used to make digest of blood?
  1. Amylase
  2. Lactase
  3. Lipase
  4. Pepsin
  5. Trypsin
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9.18) CLED agar lacks electrolytes to prevent the swarming of which species?
  1. Proteus
  2. Pseudomonas
  3. Shigella
  4. Vibrio
  5. Yersinia
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9.19) What does modified Thayer-Martin agar contain that Thayer-Martin agar does not?
  1. Nystatin
  2. Peptone water
  3. Potassium dihydrogen phosphate
  4. Trimethoprim lactate
  5. Vancomycin
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9.20) In an agar slant tube, what is the poorly oxygenated area at the bottom of the tube called?
  1. Bed
  2. Butt
  3. Foot
  4. Pith
  5. Root
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9.21) Which of these are found in all agar media?
  1. Distilled water and electrolytes
  2. Gelatin and sugar
  3. Seaweed and dissolved oxygen
  4. Vitamins and blood
  5. pH indicators and gelling properties
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9.22) What type of broth is used to differentiate between aerobes and anaerobes?
  1. Glucose
  2. Lysogeny
  3. Selenite F
  4. Thioglycolate
  5. Tryptic soy
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9.23) Which of these types of agar is used to isolate and differentiate species of Salmonella and Shigella?
  1. Hektoen enteric
  2. Chocolate
  3. CLED
  4. Thayer Martin
  5. MacConkey
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9.24) Blood for blood agar plates is usually obtained from which animal?
  1. Dogs
  2. Goats
  3. Pigs
  4. Rabbits
  5. Sheep
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9.25) What is the sequence of steps for Gram staining?
  1. Primary stain, counterstain, mordant, decolourizing
  2. Primary stain, decolourizing, counterstain, mordant
  3. Primary stain, decolourizing, mordant, counterstain
  4. Primary stain, mordant, counterstain, decolourizing
  5. Primary stain, mordant, decolourizing, counterstain
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9.26) In Gram staining, what can happen if the decolourizer is left on too long?
  1. All organisms will appear colourless
  2. Gram-negative organisms will get lysed
  3. Gram-negative organisms will look gram-positive
  4. Gram-positive organisms will look gram-negative
  5. No cells will appear on the slide
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9.27) What can happen if the heat fixation step is skipped in Gram staining?
  1. All organisms will appear colourless
  2. All organisms will appear gram-negative
  3. All organisms will appear gram-positive
  4. Gram-negative organisms will appear Gram-positive, and vice versa
  5. No cells will appear on the slide
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9.28) What is the final step in acid-fast staining?
  1. Add a drop of sterile water to a clean slide
  2. Cover the smear with the counterstain
  3. Cover the smear with the primary stain
  4. Decolourize the smear with alcohol
  5. Heat fix the smear
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9.29) Which acid-fast staining method is known as the hot method?
  1. Auramine-Rhodamine
  2. Fluorochrome
  3. Kinyoun
  4. Truant
  5. Ziehl-Neelsen
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9.30) What characteristic helps identify mycobacteria?
  1. Acid-fastness
  2. Alpha hemolytic
  3. Anaerobic
  4. Gram negative
  5. Motile
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9.31) What is the term for bacterial cells used to start a new culture on a streak plate?
  1. Colonies
  2. Inoculum
  3. Isolated colonies
  4. Prime culture
  5. T cells
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9.32) Agar plates are placed upside down in the incubator to prevent:
  1. contamination from other agar plates
  2. gas bubbles from escaping
  3. moisture from accumulating on the agar surface
  4. pathogens from overgrowing
  5. the medium from drying out
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9.33) When performing a quadrant streak plate, what action must be taken after streaking each quadrant?
  1. Allow the colonies to rest for 15–20 seconds
  2. Flame the inoculating loop
  3. Incubate the plate
  4. Invert the plate
  5. Take another loopful of inoculum from the original sample
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9.34) When a quadrant streak plate is streaked correctly, which quadrant has the most cells?
  1. All quadrants should have the same number of cells
  2. Quadrant 1
  3. Quadrant 2
  4. Quadrant 3
  5. Quadrant 4
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9.35) A lab assistant is culturing a strain of Streptococcus pyogenes which produces streptolysin O. After streaking the blood plate, what special measure should the lab assistant perform to detect this hemolysin?
  1. Add methylene blue
  2. Connect the plate to a Durham tube
  3. Invert the plate 8–10 times
  4. Pour nutrient broth over the agar
  5. Stab the agar
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9.36) What is the term for bacteria that need high levels of carbon dioxide?
  1. Capnophile
  2. Carbonophile
  3. Carbophile
  4. Caseophile
  5. Coprophile
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9.37) Obligate anaerobes are killed by:
  1. carbon dioxide
  2. light
  3. nitrogen
  4. oxygen
  5. water
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9.38) Which of these is a microaerophilic environment?
  1. 10% O₂
  2. 21% O₂
  3. 35% O₂
  4. 42% O₂
  5. 55% O₂
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9.39) What is the term for an organism that does not need oxygen?
  1. Aerobe
  2. Anaerobe
  3. Antiaerobe
  4. Microaerophile
  5. Obligate aerobe
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9.40) Which media is used to transport and preserve stool specimens?
  1. Anaerobic media
  2. Blood agar
  3. Cary-Blair media
  4. Lowenstein-Jensen agar
  5. MacConkey agar
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Section 10: Clinical Chemistry

10.1) A patient has a potassium result of 7.2 mEq/L. Before reporting the result, the lab technician should:
  1. ask the nurse if the patient is taking an anticoagulant drug
  2. check the age of the patient
  3. check the serum for bacterial contamination
  4. check the serum for hemolysis
  5. test the serum for ketones
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10.2) What is the initial screening test for Cushing syndrome?
  1. C-reactive protein test
  2. Comprehensive metabolic panel
  3. Erythrocyte sedimentation rate
  4. TSH test
  5. Urinary free cortisol test
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10.3) Which chemical does the urine glucose dipstick test use?
  1. Ferricyanide
  2. Glucose oxidase
  3. Glucose oxide
  4. Glucose reductase
  5. Hexichloridine
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10.4) Which of these tests requires a 24-hour urine specimen?
  1. A1C
  2. Creatinine clearance
  3. Glucose tolerance
  4. HCG detection
  5. Urine cytology
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10.5) Which type of urine specimen is taken after eating?
  1. 24-hour
  2. Clean catch midstream
  3. Pediatric
  4. Postprandial
  5. Random
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10.6) Which test can rule out a false positive result for bilirubin on a urine strip?
  1. Acetest
  2. Clinitest
  3. Ictotest
  4. Sulfosalicylic acid (SSA)
  5. TCA
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10.7) Midstream urine samples are most often used for:
  1. Bence-Jones protein
  2. culture and susceptibility
  3. mononucleosis testing
  4. pregnancy testing
  5. routine urinalysis
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10.8) Which of these statements about 24-hour urine collections is true?
  1. If the container contains a preservative, the preservative must be thrown away
  2. If the patient leaves their house, they should take the container with them
  3. Night-time specimens are discarded
  4. The patient must fast during the 24 hours
  5. The patient should keep the container in a warm place
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10.9) Glucose point-of-care testing screens for:
  1. glycemia
  2. glycogenesis
  3. glycogenolysis
  4. glycolysis
  5. glycosuria
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10.10) Which sample is needed for a cardiac enzyme test?
  1. Blood
  2. Sputum
  3. Stool
  4. Sweat
  5. Urine
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10.11) Which of these tests should be kept away from the light?
  1. Calcium
  2. Magnesium
  3. Magnesium
  4. Vitamin A
  5. Vitamin D
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10.12) What colour is the urine of patients with jaundice?
  1. Brownish-yellow
  2. Clear
  3. Milky
  4. Red
  5. Straw-coloured
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10.13) If a patient's urine is positive for ketones, the patient probably has:
  1. advanced liver disease
  2. heart disease
  3. pernicious anemia
  4. uncontrolled diabetes
  5. uremic syndrome
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10.14) Which of these is normally present in urine?
  1. Bilirubin
  2. Blood
  3. Creatinine
  4. Glucose
  5. Protein
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10.15) A 24-hour urine volume of 4 litres indicates:
  1. anuria
  2. nocturia
  3. oliguria
  4. polyuria
  5. pyuria
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10.16) What is the normal range for urine specific gravity?
  1. 1.003–1.035
  2. 2.003–2.035
  3. 3.003–3.035
  4. 4.003–4.035
  5. 5.003–5.035
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10.17) Adults normally produce around how many litres of urine per day?
  1. 1 to 2
  2. 3 to 4
  3. 5 to 6
  4. 7 to 8
  5. 9 to 10
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10.18) What is the standard range for blood glucose levels two hours into the oral glucose tolerance test?
  1. <2.2 mmol/L
  2. <4.4 mmol/L
  3. <7.8 mmol/L
  4. <10.0 mmol/L
  5. <13.3 mmol/L
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10.19) What is the normal range for blood bicarbonate levels in adults?
  1. 3–9 mmol/L
  2. 13–19 mmol/L
  3. 23–29 mmol/L
  4. 33–39 mmol/L
  5. 43–49 mmol/L
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10.20) What is the normal range for the blood urea nitrogen (BUN) test?
  1. 1.8–7.1 mmol/L
  2. 7.1–12.9 mmol/L
  3. 13–21 mmol/L
  4. 21–29 mmol/L
  5. 29–36 mmol/L
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10.21) What is the normal range for 24-hour urine volume?
  1. 12–24 mL
  2. 40–80 mL
  3. 100–200 mL
  4. 200–800 mL
  5. 800–2,000 mL
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10.22) What is the normal range for haptoglobin levels in blood?
  1. 0.3–2.0 g/L
  2. 2.3–4.0 g/L
  3. 4.3–6.0 g/L
  4. 6.3–8.0 g/L
  5. 8.3–10.0 g/L
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10.23) Which of these blood test results is abnormal and could indicate dehydration?
  1. Glucose: 5.3 mmol/L
  2. BUN: 5.4 mmol/L
  3. Chloride: 135 mmol/L
  4. Sodium: 140 mmol/L
  5. Creatinine: 88.4 µmol/L
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10.24) What is the normal range for fasting glucose?
  1. 0.0–2.8 mmol/L
  2. 2.8–3.9 mmol/L
  3. 3.9–5.6 mmol/L
  4. 5.6–6.9 mmol/L
  5. 6.9–8.3 mmol/L
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10.25) What is the normal range for partial pressure of carbon dioxide (pCO₂) in blood?
  1. 5–14 mmHg
  2. 15–24 mmHg
  3. 25–34 mmHg
  4. 35–44 mmHg
  5. 45–54 mmHg
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10.26) What is the normal ratio of bicarbonate to carbonic acid in blood?
  1. 20:1
  2. 80:1
  3. 200:1
  4. 800:1
  5. 1000:1
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10.27) What is the normal range for total T4 in adult blood?
  1. 1.1–2.9 nmol/L
  2. 3.3–4.7 nmol/L
  3. 18–34 nmol/L
  4. 35–59 nmol/L
  5. 64–154 nmol/L
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10.28) Which of these thyroid results is abnormal for an adult?
  1. Free T3: 5.1 pmol/L
  2. Free T4: 16 pmol/
  3. TSH: 1.5 mU/L
  4. Total T3: 5.2 nmol/L
  5. Total T4: 108 nmol/L
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10.29) Which of these liver function panel results is abnormal?
  1. ALT: 12 IU/L
  2. AST: 20 IU/L
  3. ALP: 14 IU/L
  4. GGT: 25 IU/L
  5. Bilirubin: 10 µmol/L
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10.30) Blood ammonia levels are usually measured to evaluate the health of which organ?
  1. Digestive tract
  2. Heart
  3. Liver
  4. Lungs
  5. Pancrceas
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10.31) If a blood sample has high levels of urea, it is also likely to have high levels of:
  1. albumin
  2. catalase
  3. creatinine
  4. fibrinogen
  5. lactose
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10.32) Estrogen and progesterone receptor testing are used to assess the prognosis and guide the treatment of:
  1. amenorrhea
  2. breast cancer
  3. endometriosis
  4. hepatoma
  5. ovarian cancer
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10.33) Which enzyme, found mainly in skeletal muscle, the heart, and the brain, is associated with muscle damage and myocardial infarction?
  1. ALT
  2. AST
  3. Creatine kinase
  4. Lactate dehydrogenase
  5. Lipase
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10.34) A patient has low cortisol. After an adrenocorticotropic hormone (ACTH) injection, the patient's cortisol levels are still low. The patient most likely has:
  1. Addison's disease
  2. Conn’s syndrome
  3. Cretinism
  4. Cushing‘s syndrome
  5. Cushing’s disease
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10.35) Therapeutic drug monitoring is typically performed for medications that:
  1. are long-acting
  2. are potentially addictive
  3. are short-acting
  4. have a narrow therapeutic range
  5. have high rates of nonadherence
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10.36) A blood sample has an elevated result when tested with the Jaffe reaction. This indicates:
  1. arrhythmia
  2. kidney function impairment
  3. liver disease
  4. pregnancy
  5. prolonged hypothermia
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10.37) An anti-cyclic citrullinated peptide test is usually ordered with which other test?
  1. Anti-double-stranded DNA test
  2. Aspartate aminotransferase test
  3. Factor V Leiden test
  4. Fasting glucose test
  5. Rheumatoid factor test
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Section 11: Clinical Hematology

11.1) Which colour vacutainer tube is called the erythrocyte sedimentation rate tube?
  1. Black
  2. Green
  3. Red
  4. White
  5. Yellow
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11.2) What is the normal hematocrit range for women?
  1. 16–28%
  2. 26–38%
  3. 36–48%
  4. 46–58%
  5. 56–68%
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11.3) In what unit is erythrocyte sedimentation rate reported?
  1. Millimetres cubed
  2. Millimetres per hour
  3. Millimoles per litre
  4. Nanograms per decilitre
  5. Picograms per second
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11.4) What colour is lipemic plasma?
  1. Brown
  2. Brownish-yellow
  3. Clear red
  4. Milky
  5. Straw-coloured
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11.5) Why is ammonium oxalate added to blood samples before platelet counts?
  1. To force the blood to clot
  2. To lyse the red blood cells
  3. To prevent hemodilution
  4. To prevent macrophages from destroying the platelets
  5. To stain the platelets blue
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11.6) EDTA is a(n):
  1. anticoagulant
  2. clot activator
  3. glucose preservative
  4. lactose preservative
  5. plasma separator
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11.7) What is the term for the yellow discolouration of the skin and eyes due to high bilirubin levels?
  1. Hematocrit
  2. Hemolysis
  3. Icterus
  4. Intergeneric
  5. Lipemia
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11.8) Calculate the corrected WBC count from the following results.
Test Result
Uncorrected WBC count 30,000/uL
Nucleated RBC/100 WBC 100
  1. 500/uL
  2. 10,000/uL
  3. 15,000/uL
  4. 18,000/uL
  5. 20,000/uL
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11.9) Which of these is a coagulation test?
  1. Erythrocyte sedimentation rate
  2. Full blood count
  3. Hematocrit
  4. Prothrombin time
  5. Thyroid function test
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11.10) Bleeding time evaluates the activity of:
  1. factor XIII
  2. fibrinogen
  3. labile factor
  4. platelets
  5. prothrombin
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11.11) Hematocrit results will be low for patients with:
  1. an infection
  2. anemia
  3. dehydration
  4. heart disease
  5. polycythemia vera
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11.12) Bromocresol purple and bromocresol green are dyes used to measure the levels of:
  1. Bence Jones protein
  2. albumin
  3. globulins
  4. immunoproteins
  5. light chains
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11.13) When setting up erythrocyte sedimentation rates, take care to ensure:
  1. a fasting specimen is used
  2. the ESR tubes are vertical
  3. the blood is well clotted
  4. the sample is completely thawed
  5. the water bath is exactly 37°C
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11.14) A lab technician dilutes a blood sample with tryptan blue by a ratio of 1:1. The technician then counts 100 cells in 5 of the large squares using a hemocytometer. What is the cell concentration?
  1. 2×10⁴ cells/mL
  2. 3×10⁴ cells/mL
  3. 4×10⁵ cells/mL
  4. 5×10⁵ cells/mL
  5. 6×10⁵ cells/mL
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11.15) Hematology analyzers use which principle for counting red blood cells?
  1. Campbell
  2. Coulter
  3. Loughty
  4. Stewart
  5. Thompson
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11.16) A buffy coat forms when:
  1. an SST is left to rest for 24 hours
  2. whole anticoagulated blood is centrifuged
  3. whole blood is left to rest for 24 hours
  4. whole blood is stirred with a glass rod
  5. whole coagulated blood is centrifuged
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11.17) Which stain differentiates types of blood cells?
  1. Acid-fast
  2. Gram
  3. H&E
  4. Papanicolaou
  5. Romanowsky
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11.18) Which of these is a sign of a poorly prepared blood smear?
  1. Covers the majority of the slide
  2. Fills half to three-quarters the length of the slide
  3. Has a consistent thickness throughout
  4. Has feathered edges
  5. Has space at the lateral edges
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11.19) A patient has a leukocyte count of 2.9 × 10⁹/L. What is the term for this type of cell count?
  1. Leukemia
  2. Leukocytosis
  3. Leukodystrophy
  4. Leukopenia
  5. Leukopoiesis
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11.20) What is the term for a platelet count of 120 × 10⁹/L?
  1. Anemia
  2. Neutropenia
  3. Polycythemia
  4. Thrombocytopenia
  5. Thrombocytosis
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11.21) A deficiency of red blood cells is called:
  1. anemia
  2. erythremia
  3. hemophilia
  4. leukemia
  5. neutropenia
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11.22) What does the prothrombin time test evaluate?
  1. Blood clotting
  2. Blood glucose levels
  3. Gut bacteria
  4. Kidney function
  5. Liver function
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11.23) How often should you blot the blood during a bleeding time test?
  1. Every 10 seconds
  2. Every 30 seconds
  3. Every minute
  4. Every 2 minutes
  5. Every 5 minutes
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11.24) In the prothrombin time test, the patient's plasma is mixed with:
  1. calcium and activator
  2. calcium and tissue factor
  3. glass beads
  4. platelet lipids and prothrombin
  5. platelet lipids and thrombin
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11.25) Along with the patient's prothrombin time and the average prothrombin time of the reference range population, what other information is needed to calculate a patient's international normalized ratio?
  1. The dosage of the patient's anticoagulant medication
  2. The half-life of the patient's anticoagulant medication
  3. The international sensitivity index of the thromboplastin reagent
  4. The patient's age
  5. The total thrombus volume from the thromboelastograph waveform
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11.26) What formula calculates red blood cell distribution width?
  1. 1 SD of the MCV / MCV
  2. HCT / RBC
  3. HGB / Hct
  4. HGB / RBC
  5. RBC × MCV
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11.27) What does a wide distribution curve on a blood cell histogram indicate?
  1. The blood cells are all roughly the same size
  2. The blood cells are larger than normal
  3. The blood cells are smaller than normal
  4. The blood cells are unequal in size
  5. There are more blood cells than normal
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11.28) On blood cell histograms, what does the y-axis represent?
  1. Cell colour
  2. Cell shape
  3. Cell size
  4. Number of cells
  5. Time
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11.29) Which red blood cell index is the average amount of hemoglobin in a single red blood cell?
  1. CBC
  2. MCH
  3. MCV
  4. RBC
  5. RDW
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11.30) Which of these complete blood count values is calculated, not measured?
  1. Hemoglobin
  2. MCHC
  3. Platelet count
  4. Red blood cell count
  5. White blood cell count
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11.31) A patient has the following hematology results:
Test Result
RBC 4.68×10¹²/L
Hemoglobin 133 g/L
Hematocrit 0.451
Calculate the patient's mean corpuscular hemoglobin (MCH).
  1. 0.035 pg
  2. 10.4 pg
  3. 28.4 pg
  4. 59.85 pg
  5. 295.6 pg
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11.32) What colour do red blood cells appear when stained with a Romanowsky stain?
  1. Blue
  2. Green
  3. Purple
  4. Red
  5. Yellow
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11.33) A hematology analyzer has produced results inconsistent with a patient's symptoms. What would be the most appropriate action to take first?
  1. Check that the test was run correctly
  2. Contact the manufacturer of the analyzer
  3. Inform the physician about the results
  4. Report the results but with a note about the inconsistency
  5. Request a new sample from the patient
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11.34) What visual sign suggests a plasma sample is hemolyzed?
  1. Air bubbles
  2. Blood clots
  3. Cherry red colour
  4. Decreased transparency
  5. Yellow colour
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11.35) What is the normal adult range for leukocyte count?
  1. 0.5–7.0 × 10⁹/L
  2. 2.5–9.0 × 10⁹/L
  3. 4.5–11.0 × 10⁹/L
  4. 6.5–13.0 × 10⁹/L
  5. 8.5–14.0 × 10⁹/L
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11.36) Which of these red blood cell counts is normal?
  1. 2.2 × 10¹²/L
  2. 4.9 × 10¹²/L
  3. 8.9 × 10¹²/L
  4. 10.3 × 10¹²/L
  5. 12.6 × 10¹²/L
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11.37) The normal adult range for platelet count is ______________ per nanolitre of blood.
  1. 150–450
  2. 250–550
  3. 350–650
  4. 450–750
  5. 550–850
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11.38) The normal number of white blood cells in blood is between ____________ per microlitre.
  1. 120 and 180
  2. 4,000 and 11,000
  3. 13,000 and 19,000
  4. 50,000 and 90,000
  5. 1 million and 3 million
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11.39) Which of these results indicates anemia in a male patient?
  1. Hematocrit: 36%
  2. MCH: 32 pg
  3. MCHC: 34 g/dL
  4. MCV: 90 80 fL
  5. RBC: 5.9 × 10¹²/L
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11.40) What is the normal pH range for arterial blood?
  1. 7.35–7.45
  2. 8.35–8.45
  3. 9.35–9.45
  4. 10.35–10.45
  5. 11.35–11.45
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11.41) What is a normal INR for healthy individuals?
  1. 1.1 and below
  2. 1.5–2.1
  3. 2.9–4.2
  4. 4.6–6.2
  5. 6.5 and above
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11.42) The liquid that separates out when blood clots is:
  1. fibrinogen
  2. lymph
  3. plasma
  4. serum
  5. the buffy coat
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Section 12: Transfusion Medicine

12.1) At what temperature is the incubation phase of the indirect antiglobulin test?
  1. 22°C
  2. 37°C
  3. 56°C
  4. 100°C
  5. 112°C
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12.2) Washed red blood cells are prepared by washing red cells with:
  1. acetic acid
  2. ethanol
  3. hydrochloric acid
  4. normal saline
  5. sodium hydroxide
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12.3) Which blood group system is the most important for blood transfusions?
  1. ABO
  2. Lutheran
  3. MNS
  4. P
  5. Rh
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12.4) An AB− patient requires a blood transfusion but no AB− is available. Blood from which of these blood types could be given to the patient instead?
  1. A+
  2. AB+
  3. A−
  4. B+
  5. O+
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12.5) A patient at risk for transfusion-associated graft-versus-host disease needs a blood transfusion of red blood cells. The red blood cells for this patient should be:
  1. centrifuged
  2. frozen and deglycerolized
  3. heated
  4. irradiated
  5. saline washed
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12.6) People with O− blood can only receive blood that is:
  1. AB+
  2. AB−
  3. A−
  4. B+
  5. O−
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12.7) When donated blood is bright yellow to brown, it is probably due to:
  1. bacterial contamination
  2. fibrin strands
  3. high levels of bilirubin
  4. high levels of fat
  5. red blood cells
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12.8) While separating the components of a blood donation, you notice an unusual green colour in the plasma bag. Which of these could be the cause of the green colour?
  1. The donor ate a fatty meal before the blood donation
  2. The donor ate a large number of carrots before the blood donation
  3. The donor is on a contraceptive pill
  4. The donor is taking a vitamin A supplement
  5. The plasma has red blood cells in it
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12.9) Which donated blood component carries the highest risk for bacterial contamination?
  1. Cryoprecipitate
  2. Fresh frozen plasma
  3. Plasma
  4. Platelets
  5. Red blood cells
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12.10) Fresh frozen plasma is stored at ____ or colder.
  1. −30°C
  2. −10°C
  3. −6°C
  4. 6°C
  5. 37°C
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12.11) What does "Rhesus positive" mean?
  1. The antibodies in a pregnant woman's blood are destroying her baby's blood cells
  2. The patient has a deficiency of Factor VIII, resulting in hemophilia
  3. The patient has subtype of HIV called the Rhesus subtype
  4. The patient has the D antigen on the surface of their red blood cells
  5. The patient has the rhesus antibody in their blood
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12.12) Type A antigens react with which type of antibody?
  1. Anti-A
  2. Anti-B
  3. Anti-C
  4. Anti-D
  5. Anti-E
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12.13) Why is blood type O− the universal donor?
  1. Most people are type O−
  2. O− red blood cells have no A, B or Rh antigens
  3. O− red blood cells only have O antigens
  4. O− serum has no A, B, or Rh antibodies
  5. O− serum only has O antibodies
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12.14) In blood typing, anti-A may be dyed what colour as a quality control measure?
  1. Blue
  2. Green
  3. Purple
  4. Red
  5. Yellow
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Section 13: Electrocardiograms

13.1) A continuous ECG monitor is used most commonly in a:
  1. assisted-living centre
  2. blood bank
  3. clinic
  4. hospital
  5. physician's office
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13.2) You have connected a Holter monitor to a patient. Which of these actions would next be appropriate?
  1. Give the patient a bath
  2. Have the patient start a brisk walk on a treadmill
  3. Instruct the patient to keep a diary of activities and symptoms
  4. Shave the patient's chest hair
  5. Tell the patient to lie down and keep still
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13.3) When preparing a patient for an ECG, where should you place the V1 electrode?
  1. Arm
  2. Chest
  3. Foot
  4. Hand
  5. Leg
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13.4) Leads II, III, and aVF of an ECG are called the __________ leads.
  1. extremity
  2. grounding
  3. inferior
  4. lateral
  5. limb
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13.5) The right leg (RL) electrode of the electrocardiogram is also known as:
  1. F
  2. N
  3. S
  4. T
  5. V1
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13.6) Einthoven's law states:
  1. Lead I + Lead II = Lead III
  2. Lead I + Lead III = Lead II
  3. Lead I - Lead II = Lead III
  4. Lead I - Lead III = Lead II
  5. Lead II - Lead I = Lead III
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13.7) Lead I of an ECG records differences between which electrodes?
  1. LA and LL
  2. LA and RA
  3. LA and RL
  4. RA and LL
  5. RA and RL
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13.8) How many electrodes does the 12-lead ECG use?
  1. 6
  2. 8
  3. 10
  4. 12
  5. 16
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13.9) ECG leads I, II, and III are called the _________ limb leads.
  1. augmented
  2. bipolar
  3. negative
  4. positive
  5. unipolar
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13.10) What is the normal paper speed for an ECG?
  1. 25 mm/second
  2. 50 mm/second
  3. 75 mm/second
  4. 100 mm/second
  5. 125 mm/second
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13.11) A patient is undergoing a cardiac treadmill stress test when he starts showing signs of cardiac ischemia. What should you do?
  1. Ask the patient if he can keep going
  2. Do nothing and continue to monitor the patient
  3. Slow down the treadmill to a more comfortable speed
  4. Speed up the treadmill
  5. Stop the test
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13.12) Why do ECG electrodes have tabs?
  1. So the nurse can write notes on them
  2. To attach alligator clips
  3. To attach the electrode to the patient's skin
  4. To prevent patient shocks
  5. To pull the electrode off in case of an emergency
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13.13) Why is paste or jelly applied to a patient before an ECG?
  1. To facilitate conductivity between the skin and the electrode
  2. To prevent the electrodes from overheating
  3. To reduce the risk of damaging equipment
  4. To reduce the risk of shocking the patient
  5. To reduce the risk of shocking the technician
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13.14) How long is a normal standardization mark on an ECG tracing?
  1. 10 mm
  2. 15 mm
  3. 20 mm
  4. 25 mm
  5. 30 mm
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13.15) When applying chest leads for an electrocardiogram, where is V1 placed?
  1. At the fifth intercostal space, left midclavicular line
  2. At the fourth intercostal space, left sternal border
  3. At the fourth intercostal space, right sternal border
  4. Midway between V3 and V5
  5. Near the right shoulder, close to the junction of the right arm and torso.
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13.16) When applying chest leads for an electrocardiogram, where is V2 placed?
  1. At the fifth intercostal space to the left of the midclavicular line
  2. At the fifth intercostal space to the right of the midclavicular line
  3. At the fourth intercostal space to the left of the sternum
  4. At the fourth intercostal space to the right of the sternum
  5. Midway between V3 and V5
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13.17) To prevent disease transmission during an ECG, you should:
  1. apply plenty of gel to the patient's chest
  2. check the ground prong
  3. lower the bed
  4. raise the side rail
  5. wash your hands
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13.18) In the IEC (International Electrotechnical Commission) system of ECG lead colour coding, what colour is the neutral electrode?
  1. Black
  2. Green
  3. Red
  4. White
  5. Yellow
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13.19) Lead III of an ECG records differences between the:
  1. left leg and left arm electrodes
  2. left leg and right arm electrodes
  3. right leg and chest electrodes
  4. right leg and left arm electrodes
  5. right leg and right arm electrodes
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13.20) In electrocardiography, what is the angle of Louis also known as?
  1. Anterior-posterior angle
  2. Einthoven's triangle
  3. Lead I
  4. Posterior triangle
  5. Sternal angle
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13.21) Which lead of an electrocardiogram is needed for a rhythm strip?
  1. I
  2. II
  3. III
  4. IV
  5. V
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13.22) What does the T wave on an ECG represent?
  1. Depolarization of the atria
  2. Depolarization of the ventricles
  3. Relaxation of the atria
  4. Repolarization of the atria
  5. Repolarization of the ventricles
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13.23) What does the horizontal axis on an ECG represent?
  1. Blood pressure
  2. Current
  3. Resistance
  4. Time
  5. Voltage
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13.24) What does the vertical axis on electrocardiogram paper represent?
  1. Acceleration
  2. Deacceleration
  3. Speed
  4. Time
  5. Voltage
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13.25) The S-T segment on ECG tracing represents the time interval between:
  1. atrial depolarization and atrial repolarization
  2. atrial depolarization and ventricular depolarization
  3. atrial depolarization and ventricular repolarization
  4. ventricular depolarization and atrial repolarization
  5. ventricular depolarization and ventricular repolarization
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13.26) While performing an ECG, you notice that the R wave on lead I has a negative deflection instead of a positive deflection. This means:
  1. the V1 electrode has come loose
  2. the chest leads are not attached correctly
  3. the limb leads are reversed
  4. the patient is has a pacemaker
  5. there is electrical interference coming from another machine in the room
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13.27) What causes somatic tremors on an ECG?
  1. Incorrect placement of leads
  2. Interference from another electrical device
  3. Irregular heart rate
  4. Leakage of electrical current
  5. Patient movement
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13.28) If there are 9 QRS complexes on a six-second ECG strip, then what is the patient's estimated heart rate in beats per minute?
  1. 15 bpm
  2. 54 bpm
  3. 90 bpm
  4. 154 bpm
  5. 164 bpm
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13.29) Which of these would NOT cause artifacts on an ECG?
  1. Body lotion or cream on the patient's skin
  2. Coins in the cardiologist's pocket
  3. Electrical interference
  4. Loose wires
  5. Patient movement
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13.30) An interrupted baseline on an ECG may be caused by:
  1. a broken cable
  2. electrical interference
  3. jewelry or a watch worn by the patient
  4. lotion on the patient's skin
  5. patient movement
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13.31) While recording a 12-lead ECG, you notice leads I and II have a lot of artifacts. Which electrode is probably not placed appropriately?
  1. Left arm
  2. Left leg
  3. Right arm
  4. Right leg
  5. V1
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13.32) What artifact is in this ECG tracing?
  1. AC interference
  2. Baseline wander
  3. CPR compression
  4. Loose lead
  5. Muscle tremor
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